Product: TRP1 Antibody
Catalog: DF13325
Description: Rabbit polyclonal antibody to TRP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60kDa; 61kD(Calculated).
Uniprot: P17643
RRID: AB_2846344

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
TRP1 Antibody detects endogenous levels of total TRP1.
RRID:
AB_2846344
Cite Format: Affinity Biosciences Cat# DF13325, RRID:AB_2846344.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5; 5,6 dihydroxyindole 2 carboxylic acid oxidase; 6-dihydroxyindole-2-carboxylic acid oxidase; b-PROTEIN; CAS2; Catalase B; CATB; DHICA oxidase; Glycoprotein 75; GP75; Melanoma antigen gp75; OCA3; TRP; TRP-1; TRP1; Tyrosinase related protein 1; Tyrosinase-related protein 1; TYRP; TYRP1; TYRP1_HUMAN; TYRRP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P17643 TYRP1_HUMAN:

Pigment cells.

Sequence:
MSAPKLLSLGCIFFPLLLFQQARAQFPRQCATVEALRSGMCCPDLSPVSGPGTDRCGSSSGRGRCEAVTADSRPHSPQYPHDGRDDREVWPLRFFNRTCHCNGNFSGHNCGTCRPGWRGAACDQRVLIVRRNLLDLSKEEKNHFVRALDMAKRTTHPLFVIATRRSEEILGPDGNTPQFENISIYNYFVWTHYYSVKKTFLGVGQESFGEVDFSHEGPAFLTWHRYHLLRLEKDMQEMLQEPSFSLPYWNFATGKNVCDICTDDLMGSRSNFDSTLISPNSVFSQWRVVCDSLEDYDTLGTLCNSTEDGPIRRNPAGNVARPMVQRLPEPQDVAQCLEVGLFDTPPFYSNSTNSFRNTVEGYSDPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYTYEIQWPSREFSVPEIIAIAVVGALLLVALIFGTASYLIRARRSMDEANQPLLTDQYQCYAEEYEKLQNPNQSVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
88
Chicken
88
Rabbit
88
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17643 As Substrate

Site PTM Type Enzyme
S46 Phosphorylation
N96 N-Glycosylation
N104 N-Glycosylation
S137 Phosphorylation
N181 N-Glycosylation
S207 Phosphorylation
T222 Phosphorylation
S270 Phosphorylation
N304 N-Glycosylation
N350 N-Glycosylation
N385 N-Glycosylation

Research Backgrounds

Function:

Plays a role in melanin biosynthesis. Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+). May regulate or influence the type of melanin synthesized. Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).

PTMs:

Glycosylated.

Subcellular Location:

Melanosome membrane>Single-pass type I membrane protein. Melanosome.
Note: Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Pigment cells.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the tyrosinase family.

Research Fields

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). Ding Q et al. The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine 2021 Sep 21;S0891-5849(21)00737-1. (PubMed: 34560247) [IF=7.4]

2). Hao Y et al. Bioengineered microglia-targeted exosomes facilitate Aβ clearance via enhancing activity of microglial lysosome for promoting cognitive recovery in Alzheimer's disease. Biomaterials Advances 2022 May;136:212770 (PubMed: 35929330)

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