Phospho-p70 S6 Kinase beta (Ser423) Antibody

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Product:	Phospho-p70 S6 Kinase beta (Ser423) Antibody
Catalog:	AF3486
Description:	Rabbit polyclonal antibody to Phospho-p70 S6 Kinase beta (Ser423)
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	50~70kD; 53kD(Calculated).
Uniprot:	Q9UBS0
RRID:	AB_2834924

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

Phospho-p70 S6 Kinase beta (Ser423) Antibody detects endogenous levels of p70 S6 Kinase beta only when phosphorylated at Serine 423.

RRID:

AB_2834924
Cite Format: Affinity Biosciences Cat# AF3486, RRID:AB_2834924.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; KS6B2_HUMAN; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6K 2; P70S6K2; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; Rps6kb2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K-beta-2; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B;

Immunogens

Immunogen:

Uniprot:

Q9UBS0(KS6B2_HUMAN) >>Visit HPA database.

Gene(ID):

RPS6KB2(6199)

Description:

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B).

Sequence:

Q9UBS0 KS6B2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADVQRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSIKEGFSFQPKLRSPRRLNSSPRAPVSPLKFSPFEGFRPSPSLPEPTELPLPPLLPPPPPSTTAPLPIRPPSGTKKSKRGRGRPGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Horse

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBS0 As Substrate

Q9UBS0

Site	PTM Type	Enzyme	Source
S15	Phosphorylation		Uniprot
S24	Phosphorylation		Uniprot
Y45	Phosphorylation	P12931 (SRC)	Uniprot
K75	Ubiquitination		Uniprot
K80	Ubiquitination		Uniprot
K86	Ubiquitination		Uniprot
K94	Ubiquitination		Uniprot
Y96	Phosphorylation		Uniprot
K111	Ubiquitination		Uniprot
Y136	Phosphorylation		Uniprot
K196	Ubiquitination		Uniprot
S219	Phosphorylation		Uniprot
T226	Phosphorylation		Uniprot
T228	Phosphorylation	O15530 (PDPK1)	Uniprot
S254	Phosphorylation		Uniprot
S266	Phosphorylation		Uniprot
T270	Phosphorylation		Uniprot
K276	Acetylation		Uniprot
K280	Ubiquitination		Uniprot
K285	Ubiquitination		Uniprot
S356	Phosphorylation		Uniprot
T388	Phosphorylation	P42345 (MTOR)	Uniprot
K407	Ubiquitination		Uniprot
S410	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
S417	Phosphorylation		Uniprot
S423	Phosphorylation		Uniprot
S473	Phosphorylation	P05771-2 (PRKCB) , P05129 (PRKCG) , Q05513 (PRKCZ) , P41743 (PRKCI) , Q05655 (PRKCD) , Q02156 (PRKCE) , P17252 (PRKCA)	Uniprot

PTMs - Q9UBS0 As Enzyme

Q9UBS0

Substrate	Site	Source
O00418 (EEF2K)	S366	Uniprot
P04792 (HSPB1)	S78	Uniprot
P04792 (HSPB1)	S82	Uniprot
P05783 (KRT18)	S53	Uniprot
P09651 (HNRNPA1)	S4	Uniprot
P09651 (HNRNPA1)	S6	Uniprot
P49841 (GSK3B)	S9	Uniprot
Q05195 (MXD1)	S145	Uniprot
Q53EL6 (PDCD4)	S67	Uniprot
Q6R327 (RICTOR)	T1135	Uniprot

Research Backgrounds

Q9UBS0_KS6B2_HUMAN

Function:

Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.

PTMs:

Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.

Subcellular Location:

Cytoplasm. Nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

References

1). Notch1/Hes1‑PTEN/AKT/IL‑17A feedback loop regulates Th17 cell differentiation in mouse psoriasis‑like skin inflammation. Molecular Medicine Reports, 2022 (PubMed: 35582997) [IF=3.4]

Application: WB Species: mouse Sample: skin

Figure 6. - Protein levels of (A) Notch1, (B) NICD1, (C) hairy and enhancer of split 1, (D) PTEN, (E-1) p-AKT (Thr308), (E-2) AKT, (E-3) p-AKT (Ser473), (F-1) p-mTOR (Ser2448), (F-2) mTORC1, (G) S6K1 (p70S6Kα), (H) S6K2 (p70S6Kβ) and (I) IL-17A in skin samples of experimental mice. *P

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-p70 S6 Kinase beta (Ser423) Antibody - #AF3486