Phospho-IKB beta (Ser19) Antibody | Affinity Biosciences

WB
IF/ICC
pElisa

1/3

Western blot analysis of I kappaB beta phosphorylation expression in TNF-a treated 293 whole cell lysates,The lane on the left was treated with the antigen-specific peptide.

AF3448 staining Hela cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF3448.

Product:	Phospho-IKB beta (Ser19) Antibody
Catalog:	AF3448
Description:	Rabbit polyclonal antibody to Phospho-IKB beta (Ser19)
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	37kDa; 38kD(Calculated).
Uniprot:	Q15653
RRID:	AB_2834843

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

Phospho-IKB beta (Ser19) Antibody detects endogenous levels of IKB beta only when phosphorylated at Ser19.

RRID:

AB_2834843
Cite Format: Affinity Biosciences Cat# AF3448, RRID:AB_2834843.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

I kappa B beta; I-kappa-B-beta; IkappaBbeta; IKB beta; IkB-B; IkB-beta; IKBB; IKBB_HUMAN; IkBbeta; NF kappa BIB; NF-kappa-B inhibitor beta; NF-kappa-BIB; Nfkbib; Thyroid receptor interacting protein 9; Thyroid receptor-interacting protein 9; TR interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9;

Immunogens

Immunogen:

Uniprot:

Q15653(IKBB_HUMAN) >>Visit HPA database.

Gene(ID):

NFKBIB(4793)

Expression:

Q15653 IKBB_HUMAN:

Expressed in all tissues examined.

Description:

kB-beta a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation.

Sequence:

Q15653 IKBB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15653 As Substrate

Q15653

Site	PTM Type	Enzyme	Source
S19	Phosphorylation	O14920 (IKBKB) , Q15418 (RPS6KA1)	Uniprot
S23	Phosphorylation	Q15418 (RPS6KA1) , O14920 (IKBKB)	Uniprot
Y161	Phosphorylation		Uniprot
T175	Phosphorylation		Uniprot
S183	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
K235	Ubiquitination		Uniprot
T239	Phosphorylation		Uniprot
T276	Phosphorylation		Uniprot
S280	Phosphorylation		Uniprot
S308	Phosphorylation		Uniprot
S313	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S315	Phosphorylation	P68400 (CSNK2A1)	Uniprot

Research Backgrounds

Q15653_IKBB_HUMAN

Function:

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

PTMs:

Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Interacts with THRB (via ligand-binding domain). Interacts with RELA and REL (By similarity). Interacts with COMMD1. Interacts with inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2.

Family&Domains:

Belongs to the NF-kappa-B inhibitor family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

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Phospho-IKB beta (Ser19) Antibody - #AF3448