DTX2 Antibody - #DF12523

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Product: DTX2 Antibody
Catalog: DF12523
Description: Rabbit polyclonal antibody to DTX2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67 kDa; 67kD(Calculated).
Uniprot: Q86UW9
RRID: AB_2845485

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DTX2 Antibody detects endogenous levels of total DTX2.
RRID:
AB_2845485
Cite Format: Affinity Biosciences Cat# DF12523, RRID:AB_2845485.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deltex 2; deltex homolog 2 (Drosophila); deltex, Drosophila, homolog of, 2; Deltex2; DTX2; DTX2_HUMAN; hDTX2; Highly similar to hPMSR6 [H.sapiens]; KIAA1528; Probable E3 ubiquitin-protein ligase DTX2; Protein deltex 2; Protein deltex-2; RING finger protein 58; RNF58; Zinc ion binding protein;

Immunogens

Immunogen:
Uniprot:

Q86UW9(DTX2_HUMAN) >>Visit HPA database.

Gene(ID):
DTX2(113878)
Sequence:
MAMAPSPSLVQVYTSPAAVAVWEWQDGLGTWHPYSATVCSFIEQQFVQQKGQRFGLGSLAHSIPLGQADPSLAPYIIDLPSWTQFRQDTGTMRAVRRHLFPQHSAPGRGVVWEWLSDDGSWTAYEASVCDYLEQQVARGNQLVDLAPLGYNYTVNYTTHTQTNKTSSFCRSVRRQAGPPYPVTTIIAPPGHTGVACSCHQCLSGSRTGPVSGRYRHSMTNLPAYPVPQHPPHRTASVFGTHQAFAPYNKPSLSGARSAPRLNTTNAWGAAPPSLGSQPLYRSSLSHLGPQHLPPGSSTSGAVSASLPSGPSSSPGSVPATVPMQMPKPSRVQQALAGMTSVLMSAIGLPVCLSRAPQPTSPPASRLASKSHGSVKRLRKMSVKGATPKPEPEPEQVIKNYTEELKVPPDEDCIICMEKLSTASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPQGKMEVLRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQCYLPDNAQGRKVLELLKVAWKRRLIFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
70
Zebrafish
63
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86UW9 As Substrate

Site PTM Type Enzyme
Y75 Phosphorylation
T153 Phosphorylation
Y156 Phosphorylation
R213 Methylation
R215 Methylation
S217 Phosphorylation
R233 Methylation
S236 Phosphorylation
Y247 Phosphorylation
K249 Acetylation
K249 Ubiquitination
S251 Phosphorylation
S253 Phosphorylation
R256 Methylation
S257 Phosphorylation
Y280 Phosphorylation
R281 Methylation
S360 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
K369 Ubiquitination
S373 Phosphorylation
K379 Acetylation
K383 Acetylation
T386 Phosphorylation
K398 Ubiquitination
K473 Ubiquitination
K479 Ubiquitination
K553 Acetylation
K553 Ubiquitination
K557 Acetylation

Research Backgrounds

Function:

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic. Partially nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1.

Family&Domains:

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.

Belongs to the Deltex family.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

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