E2F3 Antibody - #DF12390

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Product: E2F3 Antibody
Catalog: DF12390
Description: Rabbit polyclonal antibody to E2F3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 60-65 kDa; 49kD(Calculated).
Uniprot: O00716
RRID: AB_2845195

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
E2F3 Antibody detects endogenous levels of total E2F3.
RRID:
AB_2845195
Cite Format: Affinity Biosciences Cat# DF12390, RRID:AB_2845195.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686C18211; E2F 3; E2F transcription factor 3; E2F-3; E2F3; E2F3_HUMAN; KIAA0075; MGC104598; Transcription factor E2F 3; Transcription factor E2F3;

Immunogens

Immunogen:
Uniprot:

O00716(E2F3_HUMAN) >>Visit HPA database.

Gene(ID):
E2F3(1871)
Sequence:
MRKGIQPALEQYLVTAGGGEGAAVVAAAAAASMDKRALLASPGFAAAAAAAAAPGAYIQILTTNTSTTSCSSSLQSGAVAAGPLLPSAPGAEQTAGSLLYTTPHGPSSRAGLLQQPPALGRGGSGGGGGPPAKRRLELGESGHQYLSDGLKTPKGKGRAALRSPDSPKTPKSPSEKTRYDTSLGLLTKKFIQLLSQSPDGVLDLNKAAEVLKVQKRRIYDITNVLEGIHLIKKKSKNNVQWMGCSLSEDGGMLAQCQGLSKEVTELSQEEKKLDELIQSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKAPPETRLEVPDSIESLQIHLASTQGPIEVYLCPEETETHSPMKTNNQDHNGNIPKPASKDLASTNSGHSDCSVSMGNLSPLASPANLLQQTEDQIPSNLEGPFVNLLPPLLQEDYLLSLGEEEGISDLFDAYDLEKLPLVEDFMCS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00716 As Substrate

Site PTM Type Enzyme
Phosphorylation
R121 Methylation
S124 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2)
T152 Phosphorylation
S163 Phosphorylation
S166 Phosphorylation
K168 Acetylation
T169 Phosphorylation
K171 Acetylation
S172 Phosphorylation
S174 Phosphorylation
K176 Acetylation
Y179 Phosphorylation
S182 Phosphorylation
K188 Ubiquitination
K206 Ubiquitination
K212 Ubiquitination
S235 Phosphorylation
S267 Phosphorylation
K306 Acetylation
Y349 Phosphorylation

Research Backgrounds

Function:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.

Family&Domains:

Belongs to the E2F/DP family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). IGF2BP3 enhances the mRNA stability of E2F3 by interacting with LINC00958 to promote endometrial carcinoma progression. Cell Death Discovery, 2022 (PubMed: 35676262) [IF=7.0]
2). CircRHOT1 Mediated Cell Proliferation, Apoptosis and Invasion of Pancreatic Cancer Cells by Sponging miR125a-3p. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32697386) [IF=5.3]

Application: WB    Species: human    Sample: HPDE and PANC-1 cells

FIGURE 4|CircRHOT1 regulates the expression of E2F3 by targeting miR-125a-3p in PANC-1 cells.F and G, Relative expression of E2F3mRNA and protein in human pancreatic ductal cell line (HPDE) and PANC-1 cells was measured by RT-qPCR.
3). Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer. Oncology Letters, 2017 (PubMed: 29085511) [IF=2.9]

Application: WB    Species: human    Sample: A549 cells

Figure 3. |NEAT1 promotes E2F3 expression in part through competitive binding miRNA‑377‑3p. The relative level of miR‑377‑3p in A549 and H1299 (A). The luciferase report assay demonstrated that miR‑377‑3p could reduce the intensity of fluorescence in A549 (B) and H1299 (C) transfected with the NEAT1‑WT vector. Luciferase reporter assays indicated that luciferase activity generated by the reporter vector with E2F3‑3'‑WT decreased more than 50% after co‑transfected with miR‑377‑3p in both A549 (D) and H1299 (E). Overexpression of miR‑377‑3p inhibited NEAT1 expression (F). Overexpression of miR‑377‑3p inhibited E2F expression in A549 (G).

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