PFKFB3 Antibody - #DF12016

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Product: PFKFB3 Antibody
Catalog: DF12016
Description: Rabbit polyclonal antibody to PFKFB3
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 58 kDa; 60kD(Calculated).
Uniprot: Q16875
RRID: AB_2844821

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PFKFB3 Antibody detects endogenous levels of total PFKFB3.
RRID:
AB_2844821
Cite Format: Affinity Biosciences Cat# DF12016, RRID:AB_2844821.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

Immunogens

Immunogen:

A synthesized peptide derived from human PFKFB3, corresponding to a region within C-terminal amino acids.

Uniprot:

Q16875(F263_HUMAN) >>Visit HPA database.

Gene(ID):
PFKFB3(5209)
Expression:
Q16875 F263_HUMAN:

Ubiquitous.

Sequence:
MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

Research Backgrounds

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

PTMs:

Phosphorylation by AMPK stimulates activity.

Tissue Specificity:

Ubiquitous.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

References

1). Rosmarinic Acid Alleviates Radiation-Induced Pulmonary Fibrosis by Downregulating the tRNA N7-Methylguanosine Modification-Regulated Fibroblast-to-Myofibroblast Transition Through the Exosome Pathway. Journal of inflammation research, 2024 (PubMed: 39188632) [IF=4.5]

Application: WB    Species: Mouse    Sample: MLFs

Figure 7 RA diminished glycolysis by reducing acetylated PFKFB3 and cytoplasmic translocation. (A) ECARs were evaluated via a Seahorse XFe96 Extracellular Flux Analyzer. (B) The acetylation levels of PFKFB3 were examined via Western blotting. (C) After treatment with Remodelin (an inhibitor of NAT10), the acetylation levels of PFKFB3 in MLFs were examined. (D) The acetylation sites of PFKFB3 were examined by immunoprecipitation. (E) The effects of the K472Q and K473Q mutations on the distribution of PFKFB3 in MLFs were assessed via immunofluorescence staining. Scale bars, 50 μm. (F) Phosphorylation levels of PFKFB3 and AMPK were examined. (G and H) The effects of the K472Q and K473Q mutations on the phosphorylation levels of PFKFB3 were examined. (I) The effects of the K472Q and K473Q mutations on the ECAR were evaluated via the Seahorse assay.
2). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.8]

Application: WB    Species: Human    Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.
3). PFKFB3 facilitates cell proliferation and migration in anaplastic thyroid carcinoma via the WNT/β-catenin signaling pathway. Endocrine, 2024 (PubMed: 38378893) [IF=3.0]
4). Effect of exercise training on cardiac function and glucose metabolism in the ischemic border zone: insights from multi-modal imaging techniques. Frontiers in cardiovascular medicine, 2025 (PubMed: 40520940) [IF=2.8]

Application: IHC    Species: Rat    Sample: myocardial tissues

Figure 6. Effects of exercise on the expressions of GLUT4 and PFKFB3 in myocardial tissues of rats after 8 weeks of exercise training. (A,C) Representative micrographs of heart sections from the ischemic border zones of the three groups. (B,D) Protein expressional levels of GLUT4 and PFKFB3 in the Sham, MIC and MIE groups. n = 4 rats per group. ***P 
5). STAT3 signaling mediates peritoneal fibrosis by activating hyperglycolysis. American journal of translational research, 2022 (PubMed: 36398234) [IF=1.7]

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