Cyclin E1 Antibody - #AF4713

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Product: Cyclin E1 Antibody
Catalog: AF4713
Description: Rabbit polyclonal antibody to Cyclin E1
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Chicken
Mol.Wt.: 48kDa; 47kD(Calculated).
Uniprot: P24864
RRID: AB_2844717

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
Cyclin E1 Antibody detects endogenous levels of total Cyclin E1.
RRID:
AB_2844717
Cite Format: Affinity Biosciences Cat# AF4713, RRID:AB_2844717.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1;

Immunogens

Immunogen:

A synthesized peptide derived from human Cyclin E1, corresponding to a region within N-terminal amino acids.

Uniprot:

P24864(CCNE1_HUMAN) >>Visit HPA database.

Gene(ID):
CCNE1(898)
Expression:
P24864 CCNE1_HUMAN:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Sequence:
MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Chicken
89
Pig
82
Xenopus
78
Zebrafish
67
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Essential for the control of the cell cycle at the G1/S (start) transition.

PTMs:

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.

Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Family&Domains:

Belongs to the cyclin family. Cyclin E subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Communications biology, 2024 (PubMed: 39358475) [IF=5.9]

Application: WB    Species: Mouse    Sample: GCs

Fig. 2: TAB2 inhibits the apoptosis but promotes the proliferation of GCs. The mRNA (A) and protein (B) expressions of apoptosis-related genes treated with TAB2 overexpression in GCs. C The effect of TAB2 overexpression on the apoptosis of GCs. The mRNA (D) and protein (E) expressions of apoptosis-related genes treated with TAB2 knockdown in GCs. F The effect of TAB2 knockdown on the apoptosis of GCs. The mRNA (G) and protein (H) expressions of proliferation-related genes treated with TAB2 overexpression in GCs. I The effect of TAB2 overexpression on the proliferation of GCs. The mRNA (J) and protein (K) expressions of proliferation-related genes treated with TAB2 knockdown in GCs. L The effect of TAB2 knockdown on the proliferation of GCs. The scale bars in (I, L) are 200 μm.
2). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 2. SLCO3A1 promotes the cell cycle-related processes but inhibits the apoptosis of GCs. The cell cycle distribution was analyzed by flow cytometric transfected with OE-SLCO3A1 (A) or KD-SLCO3A1 (B). Green: cells in the G0-G1 phase, Yellow: cells in S phase, Blue: cells in G2-M phase. (C) RT-qPCR analysis of cycle-related genes expression transfected with OE-NC or OE-SLCO3A1. (D) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with OE-NC or OE-SLCO3A1. (E) RT-qPCR analysis of cycle-related genes expression transfected with KD-NC or KD-SLCO3A1. (F) Western blot analysis of CCNE1, CDK2 and CCND1 transfected with KD-NC or KD-SLCO3A1. Flow cytometry assay of the apoptosis rate transfected with OE-SLCO3A1 (G) or KD-SLCO3A1 (H). Q1: dead cells and cell debris, Q2: late apoptotic cells, Q3: early apoptotic cells, Q4: negative cells. RT-qPCR analysis of pro-apoptosis related genes expression transfected with OE-SLCO3A1 (I) or KD-SLCO3A1 (K). Western blot analysis of CASP3 and CASP8 transfected with OE-SLCO3A1 (J) or KD-SLCO3A1 (L). All of the above experimental results were obtained in the COV434 cells. * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05.

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