Product: Phospho-NUMB (Ser276) Antibody
Catalog: AF4435
Description: Rabbit polyclonal antibody to Phospho-NUMB (Ser276)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 78kDa; 71kD(Calculated).
Uniprot: P49757
RRID: AB_2844499

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-NUMB (Ser276) Antibody detects endogenous levels of NUMB only when phosphorylated at Ser276.
RRID:
AB_2844499
Cite Format: Affinity Biosciences Cat# AF4435, RRID:AB_2844499.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c14_5527; C14orf41; FLJ31314; h Numb; h-Numb; Numb; Numb homolog (Drosophila); Numb homolog; Numb protein homolog; NUMB_HUMAN; Protein numb homolog; Protein S171; S171;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQSPTFQANGTDSAFHVLAKPAHTALAPVAMPVRETNPWAHAPDAANKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPAQSYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
63
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49757 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
K12 Ubiquitination
Y15 Phosphorylation
S20 Phosphorylation
T27 Phosphorylation
K36 Ubiquitination
R53 Methylation
K63 Ubiquitination
K73 Ubiquitination
S91 Phosphorylation
T102 Phosphorylation Q2M2I8 (AAK1)
R121 Methylation
T187 Phosphorylation
T190 Phosphorylation
S194 Phosphorylation
S238 Phosphorylation
S240 Phosphorylation
S241 Phosphorylation
T243 Phosphorylation
S244 Phosphorylation
T246 Phosphorylation
T251 Phosphorylation
S276 Phosphorylation Q14012 (CAMK1)
R278 Methylation
S284 Phosphorylation
K286 Ubiquitination
S288 Phosphorylation Q00535 (CDK5)
S295 Phosphorylation Q14012 (CAMK1)
S361 Phosphorylation
S425 Phosphorylation
T436 Phosphorylation
S438 Phosphorylation
S448 Phosphorylation
T561 Phosphorylation
T631 Phosphorylation
S634 Phosphorylation

Research Backgrounds

Function:

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

PTMs:

Phosphorylated on Ser-276 and Ser-295 by CaMK1.

Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Subcellular Location:

Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CDH1 and TFAP2B (By similarity). Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

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