Phospho-CD32 (Tyr288) Antibody | Affinity Biosciences

IHC
pElisa

1/2

AF4348 at 1/100 staining Rat heart tissue by IHC-P.

Product:	Phospho-CD32 (Tyr288) Antibody
Catalog:	AF4348
Description:	Rabbit polyclonal antibody to Phospho-CD32 (Tyr288)
Application:	WB IHC
Reactivity:	Human, Rat
Mol.Wt.:	40kDa,44 kDa; 35kD(Calculated).
Uniprot:	P12318
RRID:	AB_2844427

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

Phospho-CD32 (Tyr288) Antibody detects endogenous levels of CD32 only when phosphorylated at Tyr288.

RRID:

AB_2844427
Cite Format: Affinity Biosciences Cat# AF4348, RRID:AB_2844427.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD32; CD32 antigen; CDw32; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RII a; Fc gamma RIIa; Fc-gamma RII-a; Fc-gamma-RIIa; FCG2; FCG2A_HUMAN; FcGR; FCGR2; FCGR2A; FCGR2A1; FcRII a; FcRII-a; IGFR2; IgG Fc receptor II a; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II a; Low affinity immunoglobulin gamma Fc region receptor II-a;

Immunogens

Immunogen:

Uniprot:

P12318(FCG2A_HUMAN) >>Visit HPA database.

Gene(ID):

FCGR2A(2212)

Expression:

P12318 FCG2A_HUMAN:

Found on monocytes, neutrophils and eosinophil platelets.

Sequence:

P12318 FCG2A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTMETQMSQNVCPRNLWLLQPLTVLLLLASADSQAAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGIIVAVVIATAVAAIVAAVVALIYCRKKRISANSTDPVKAAQFEPPGRQMIAIRKRQLEETNNDYETADGGYMTLNPRAPTDDDKNIYLTLPPNDHVNSNN

PTMs - P12318 As Substrate

P12318

Site	PTM Type	Enzyme	Source
N97	N-Glycosylation		Uniprot
N178	N-Glycosylation		Uniprot
Y240	Phosphorylation		Uniprot
K255	Ubiquitination		Uniprot
K271	Acetylation		Uniprot
T277	Phosphorylation		Uniprot
Y281	Phosphorylation	P51451 (BLK) , P43405 (SYK) , P07948 (LYN) , P06241 (FYN)	Uniprot
T283	Phosphorylation		Uniprot
Y288	Phosphorylation	P07948 (LYN) , P43405 (SYK) , P51451 (BLK) , P06241 (FYN)	Uniprot
T290	Phosphorylation		Uniprot
T297	Phosphorylation		Uniprot
Y304	Phosphorylation	P43405 (SYK) , P06241 (FYN) , P07948 (LYN) , P51451 (BLK)	Uniprot

Research Backgrounds

P12318_FCG2A_HUMAN

Function:

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

PTMs:

Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Found on monocytes, neutrophils and eosinophil platelets.

Subunit Structure:

Interacts with INPP5D/SHIP1 and INPPL1/SHIP2, regulating its function. Interacts with APCS and FGR. Interacts with HCK.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-CD32 (Tyr288) Antibody - #AF4348