Phospho-alpha-Actinin (Tyr193) Antibody - #AF4329

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Product: Phospho-alpha-Actinin (Tyr193) Antibody
Catalog: AF4329
Description: Rabbit polyclonal antibody to Phospho-alpha-Actinin (Tyr193)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 105kDa; 103kD(Calculated).
Uniprot: P12814
RRID: AB_2844408

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-alpha-Actinin (Tyr193) Antibody detects endogenous levels of alpha-Actinin only when phosphorylated at Tyr193.
RRID:
AB_2844408
Cite Format: Affinity Biosciences Cat# AF4329, RRID:AB_2844408.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

actinin 1 smooth muscle; Actinin alpha 1; actinin, alpha 1; ACTN 1; Actn1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; F actin cross linking protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non muscle alpha actinin 1; Non-muscle alpha-actinin-1;

Immunogens

Immunogen:
Uniprot:

P12814(ACTN1_HUMAN) >>Visit HPA database.

Gene(ID):
ACTN1(87)
Sequence:
MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12814 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y4 Phosphorylation
S6 Phosphorylation
T9 Phosphorylation
Y12 Phosphorylation A0A059VC25 (FAK) , Q05397 (PTK2)
K31 Ubiquitination
K35 Ubiquitination
K47 Ubiquitination
T50 Phosphorylation
S73 Phosphorylation
K89 Ubiquitination
S91 Phosphorylation
K95 Acetylation
S102 Phosphorylation
K103 Ubiquitination
S109 Phosphorylation
T123 Phosphorylation
S140 Phosphorylation
T144 Phosphorylation
S145 Phosphorylation
C154 S-Nitrosylation
K162 Ubiquitination
S172 Phosphorylation
Y193 Phosphorylation
K195 Acetylation
K195 Ubiquitination
K198 Acetylation
K198 Ubiquitination
K214 Ubiquitination
Y215 Phosphorylation
K220 Ubiquitination
T230 Phosphorylation
Y241 Phosphorylation
S243 Phosphorylation
S244 Phosphorylation
Y246 Phosphorylation
S250 Phosphorylation
R261 Methylation
Y279 Phosphorylation
S284 Phosphorylation
R291 Methylation
Y319 Phosphorylation
K324 Ubiquitination
K327 Ubiquitination
K331 Ubiquitination
C332 S-Nitrosylation
T340 Phosphorylation
T343 Phosphorylation
S348 Phosphorylation
S356 Phosphorylation
S362 Phosphorylation
Y378 Phosphorylation
K398 Acetylation
K398 Ubiquitination
K402 Ubiquitination
S404 Phosphorylation
T410 Phosphorylation
K413 Ubiquitination
Y422 Phosphorylation
T426 Phosphorylation
S428 Phosphorylation
K431 Ubiquitination
K436 Acetylation
K436 Ubiquitination
S442 Phosphorylation
Y466 Phosphorylation
Y467 Phosphorylation
S471 Phosphorylation
K478 Ubiquitination
C480 S-Nitrosylation
K492 Acetylation
K492 Ubiquitination
K502 Ubiquitination
Y511 Phosphorylation
Y514 Phosphorylation
S576 Phosphorylation
Y582 Phosphorylation
K633 Ubiquitination
S669 Phosphorylation
Y674 Phosphorylation
K676 Acetylation
K676 Ubiquitination
S677 Phosphorylation
Y681 Phosphorylation
K684 Ubiquitination
Y708 Phosphorylation
T722 Phosphorylation
T727 Phosphorylation
T737 Phosphorylation
S744 Phosphorylation
S763 Phosphorylation
T765 Phosphorylation
S797 Phosphorylation
T820 Phosphorylation
T823 Phosphorylation
T825 Phosphorylation
S832 Phosphorylation
Y842 Phosphorylation
T844 Phosphorylation
Y859 Phosphorylation
Y887 Phosphorylation
S890 Phosphorylation

Research Backgrounds

Function:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril>Sarcomere>Z line. Cell membrane. Cell junction. Cell projection>Ruffle.
Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; antiparallel. Interacts with MYOZ2, TTID and LPP. Interacts with DDN. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN. Interacts with PDLIM1. Interacts with PDLIM2. Interacts with PDLIM4 (via PDZ domain) (By similarity).

Family&Domains:

Belongs to the alpha-actinin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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