Product: RPS6 Antibody
Catalog: AF7831
Description: Rabbit polyclonal antibody to RPS6
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32kDa, 37 kDa; 29kD(Calculated).
Uniprot: P62753
RRID: AB_2844195

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
RPS6 Antibody detects endogenous levels of total RPS6.
RRID:
AB_2844195
Cite Format: Affinity Biosciences Cat# AF7831, RRID:AB_2844195.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Chicken
90
Zebrafish
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62753 As Substrate

Site PTM Type Enzyme
K2 Methylation
S6 Phosphorylation
C12 S-Nitrosylation
K14 Acetylation
K14 Ubiquitination
R22 Methylation
K23 Ubiquitination
Y28 Phosphorylation
K30 Acetylation
K30 Ubiquitination
K46 Ubiquitination
S53 Phosphorylation
K58 Ubiquitination
K64 Ubiquitination
T69 Phosphorylation
S78 Phosphorylation
K79 Acetylation
K79 Ubiquitination
S82 Phosphorylation
K116 Ubiquitination
K119 Ubiquitination
T128 Phosphorylation
K143 Ubiquitination
S148 Phosphorylation
K149 Ubiquitination
R154 Methylation
K160 Ubiquitination
T181 Phosphorylation
K203 Ubiquitination
Y209 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K218 Acetylation
S235 Phosphorylation Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1)
S236 Phosphorylation Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK)
S240 Phosphorylation Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
T241 Phosphorylation
S242 Phosphorylation Q13177 (PAK2)
S244 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S246 Phosphorylation
S247 Phosphorylation P23443 (RPS6KB1)

Research Backgrounds

Function:

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

PTMs:

Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS6 family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

References

1). Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway. Frontiers in Pharmacology, 2021 (PubMed: 33912053) [IF=5.6]

Application: WB    Species: Mice    Sample: BLM-PPF cells

FIGURE 4 Ellagic acid promotes pulmonary fibroblast autophagy mainly via inhibiting Wnt-mTOR signaling pathway (A,B) Mlg cells were exposed to CQ (20 µM) and Baf A1 (100 nM) with or without Ellagic acid (5 µM, 10 µM) to analyze the p62 expression level by using western blot. Densitometric analyses were shown beside (C,D) The plasmids of GFP-LC3B and mCherry-GFP-LC3B were transfected to NIH3T3 cells with PEI, and these cells were subsequently exposed to Ellagic acid (5 µM, 10 µM) and/or Wnt3a (100 ng/ml) for 12 h. DNA was counterstained with DAPI (blue). Quantitative analyses are showed beside. Scale bars: 50 μm (E) BLM-PPF cells were treated with Ellagic acid (5 µM, 10 µM) for 24 h, and the Atg16L1, Beclin1 and LC3-II/I expression levels were detected by Western blot. Densitometric analyses were shown below (F) BLM-PPF cells were treated with Ellagic acid (5 µM, 10 µM) for 12 h, and protein expression levels of mTOR, S6RP and their phosphorylation were detected by Western blot. Densitometric analyses were shown below. Data are means ± Standard Error of Mean, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, and NS: nonsignificant (one-way ANOVA). β-tubulin or GAPDH were used as a loading control.

Application: WB    Species: mouse    Sample: BLM-PPF cells

FIGURE 4 |Densitometric analyses were shown below (F) BLM-PPF cells were treated with Ellagic acid (5 µM, 10 µM) for 12 h, and protein expression levels of mTOR, S6RP and their phosphorylation were detected by Western blot. Densitometric analyses were shown below. Data are means ± Standard Error of Mean, n  3, *p < 0.05, **p < 0.01,***p < 0.001, and NS: nonsignificant (one-way ANOVA). β-tubulin or GAPDH were used as a loading control.

2). Regorafenib-Attenuated, Bleomycin-Induced Pulmonary Fibrosis by Inhibiting the TGF-β1 Signaling Pathway. International Journal of Molecular Sciences, 2021 (PubMed: 33671452) [IF=5.6]

Application: WB    Species: Mouse    Sample: Mlg cells

Figure 4. Regorafenib induces the formation of autophagy by inhibiting the TGF-β1/mTOR signal pathway. (A) BLM-PPF cells were treated with RG (2 µM, 4 µM) for 24 h, and the expression levels of autophagy-related proteins (Atg3, Atg7, p62, and LC3-II/I) were detected. Densitometric analysis are shown beside; (B) Mlg cells were exposed to chloroquine (CQ) and/or RG (2 µM, 4 µM) for 24 h, and Western blot technology was used to detect the p62 protein expression level; (C) Mlg cells were exposed to bafilomycin A1 (Baf A1) and/or RG (2 µM, 4 µM) for 24 h. Densitometric analysis are shown below; (D,E) The plasmids of GFP-LC3B (green fluorescent protein-microtubule-associated protein 1 light chain 3B) and mCherry-GFP-LC3B (mCherry fluorescent protein-green fluorescent protein-microtubule-associated protein 1 light chain 3B) was transferred into NIH-3T3 cells, and these cells subsequently were incubated with RG (2 µM, 4 µM) and/or TGF-β1 (5 ng/mL) for 12 h. DNA was counterstained with DAPI (blue). Scale bars: 50 µm; (F) Mlg cells were incubated with RG (2 µM, 4 µM) and/or TGF-β1 (5 ng/mL) for 12 h, and the expression levels of mTOR (mechanistic target of rapamycin), ULK-1 (protein kinase ULK1/autophagy-related protein 1), p70 S6K (p70 S6 kinase), and S6RP (S6 ribosomal protein), and their phosphorylations were detected. Densitometric analysis are shown beside; (G) BLM-PPF cells were explored to RG (2 µM, 4 µM) for 12 h, and Western Blot were used to detect the expression levels of mTOR, ULK-1, and S6RP, and their phosphorylations. Densitometric analysis are shown beside. Data in (A–G) are means ± standard error of mean (SEM), and GAPDH or β-tubulin were used as a loading control. # p < 0.05, ### p < 0.001, ** p < 0.01, *** p < 0.001(one-way ANOVA). NS: not significant.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.