Phospho-iNOS (Tyr151) Antibody - #AF3270
| Product: | Phospho-iNOS (Tyr151) Antibody |
| Catalog: | AF3270 |
| Description: | Rabbit polyclonal antibody to Phospho-iNOS (Tyr151) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 132kDa; 131kD(Calculated). |
| Uniprot: | P35228 |
| RRID: | AB_2834696 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3270, RRID:AB_2834696.
Fold/Unfold
HEP-NOS; Hepatocyte NOS; HEPNOS; inducible; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible; Nitric oxide synthase 2 inducible macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase; Nitric oxide synthase inducible; nitric oxide synthase, macrophage; NOS 2; NOS; Nos II; NOS type II; nos2; NOS2_HUMAN; NOS2A; NOS2A, Inducible, Hepatocyte; Peptidyl-cysteine S-nitrosylase NOS2;
Immunogens
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
- P35228 NOS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35228 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S50 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| Y151 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y267 | Phosphorylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y446 | Phosphorylation | Uniprot | |
| Y451 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| K604 | Acetylation | Uniprot | |
| R670 | Methylation | Uniprot | |
| S745 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| Y868 | Phosphorylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| S898 | Phosphorylation | Uniprot | |
| Y1055 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y1123 | Phosphorylation | Uniprot | |
| Y1134 | Phosphorylation | Uniprot |
Research Backgrounds
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Cytoplasm>Cytosol.
Note: Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization.
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Homodimer. Interacts with SLC9A3R1. Interacts with GAPDH; induced by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex. Interacts with SPSB1, SPSB2 and SPSB4. Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4.
Belongs to the NOS family.
Research Fields
· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Metabolism > Amino acid metabolism > Arginine biosynthesis.
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
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