IL-2RA/CD25 Antibody | Affinity Biosciences

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Western blot analysis of IL-2R /CD25 in lysates of LOVO, using IL-2R /CD25 Antibody(AF7675).

Product:	IL-2RA/CD25 Antibody
Catalog:	AF7675
Description:	Rabbit polyclonal antibody to IL-2RA/CD25
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Horse, Dog
Mol.Wt.:	31kDa; 31kD(Calculated).
Uniprot:	P01589
RRID:	AB_2844039

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(89%), Dog(86%)

Clonality:

Polyclonal

Specificity:

IL-2RA/CD25 Antibody detects endogenous levels of total IL-2RA/CD25.

RRID:

AB_2844039
Cite Format: Affinity Biosciences Cat# AF7675, RRID:AB_2844039.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Interleukin 2 receptor alpha chain; CD25; CD25 antigen; IDDM10; IL 2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2-RA; IL2R; IL2R, alpha chain; IL2RA; IL2RA_HUMAN; IMD41; Interleukin 2 receptor alpha; Interleukin 2 receptor; Interleukin-2 receptor subunit alpha; p55; t-cell growth factor receptor; TAC; TAC antigen; TCGFR;

Immunogens

Immunogen:

Uniprot:

P01589(IL2RA_HUMAN) >>Visit HPA database.

Gene(ID):

IL2RA(3559)

Sequence:

P01589 IL2RA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSYLLMWGLLTFIMVPGCQAELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQVAVAGCVFLLISVLLLSGLTWQRRQRKSRRTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01589 As Substrate

P01589

Site	PTM Type	Enzyme	Source
N70	N-Glycosylation		Uniprot
N89	N-Glycosylation		Uniprot
T218	O-Glycosylation		Uniprot
T224	O-Glycosylation		Uniprot
T229	O-Glycosylation		Uniprot
T237	O-Glycosylation		Uniprot
S268	Phosphorylation	P17252 (PRKCA)	Uniprot
T271	Phosphorylation	P17252 (PRKCA)	Uniprot

Research Backgrounds

P01589_IL2RA_HUMAN

Function:

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Subunit Structure:

Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

References

1). Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance. Journal of Translational Medicine (PubMed: 34407839) [IF=7.4]

Application: WB Species: Mouse Sample: colon tissue

Fig. 6 L.p R3 attenuates colonic inflammatory responses but induces Treg responses in DSS-induced experimental murine colitis. A The expression of Foxp3, IL-10, IL-17A and IFN-γ were detected in the colon by the immunohistochemical method. B Pathological score of Foxp3, IL-10, IL-17A and IFN-γ expression in colon tissue. C Evaluate the expression of CD4, CD25, Foxp3, IL-17A, IFN-γ, IL-10 in colon tissue by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001

2). SLC35A2 expression drives breast cancer progression via ERK pathway activation. The FEBS journal (PubMed: 38143314) [IF=5.4]

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IL-2RA/CD25 Antibody - #AF7675