Phospho-CaMKII (Ser235) Antibody - #AF7378

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Product: Phospho-CaMKII (Ser235) Antibody
Catalog: AF7378
Description: Rabbit polyclonal antibody to Phospho-CaMKII (Ser235)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.: 45,60kDa; 73kD(Calculated).
Uniprot: Q13554
RRID: AB_2843818

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CaMKII (Ser235) Antibody detects endogenous levels of CaMKII only when phosphorylated at Ser235.
RRID:
AB_2843818
Cite Format: Affinity Biosciences Cat# AF7378, RRID:AB_2843818.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit alpha; CaM kinase II subunit delta; CAM2; CaMK-II subunit alpha; CaMK-II subunit delta; CAMK2A; CAMK2B; CAMKA; CAMKB; CAMKD; CaMKII alpha; KIAA0968;

Immunogens

Immunogen:
Uniprot:

Q13554(KCC2B_HUMAN) >>Visit HPA database.

Gene(ID):
CAMK2B(816)
Expression:
Q13554 KCC2B_HUMAN:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

Sequence:
MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKAPRVPDILSSVRRGSGAPEAEGPLPCPSPAPFSPLPAPSPRISDILNSVRRGSGTPEAEGPLSAGPPPCLSPALLGPLSSPSPRISDILNSVRRGSGTPEAEGPSPVGPPPCPSPTIPGPLPTPSRKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13554 As Substrate

Site PTM Type Enzyme
K57 Ubiquitination
S79 Phosphorylation
K138 Ubiquitination
S146 Phosphorylation
K227 Acetylation
K227 Ubiquitination
Y231 Phosphorylation
S235 Phosphorylation
K246 Ubiquitination
T254 Phosphorylation
K259 Ubiquitination
K268 Ubiquitination
S276 Phosphorylation
S280 Phosphorylation
T287 Phosphorylation Q13554 (CAMK2B)
K299 Methylation
K301 Methylation
T306 Phosphorylation
T307 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation
T328 Phosphorylation
K342 Acetylation
S394 Phosphorylation
S395 Phosphorylation
S397 Phosphorylation
T400 Phosphorylation
T401 Phosphorylation
S423 Phosphorylation
S436 Phosphorylation
S441 Phosphorylation
S456 Phosphorylation
S461 Phosphorylation
T463 Phosphorylation
S471 Phosphorylation
S479 Phosphorylation
S499 Phosphorylation
S504 Phosphorylation
T506 Phosphorylation
S522 Phosphorylation

PTMs - Q13554 As Enzyme

Substrate Site Source
O14490-2 (DLGAP1) S349 Uniprot
O14490-2 (DLGAP1) S393 Uniprot
P14921-1 (ETS1) S251 Uniprot
P14921-1 (ETS1) S257 Uniprot
P14921-1 (ETS1) S282 Uniprot
P14921-1 (ETS1) S285 Uniprot
P16949 (STMN1) S16 Uniprot
P26678 (PLN) T17 Uniprot
P35222 (CTNNB1) T332 Uniprot
P35222 (CTNNB1) T472 Uniprot
P35222 (CTNNB1) S552 Uniprot
P42224 (STAT1) S727 Uniprot
P49840 (GSK3A) S21 Uniprot
Q01970-1 (PLCB3) S537 Uniprot
Q12879-1 (GRIN2A) S1291 Uniprot
Q13131 (PRKAA1) T183 Uniprot
Q13224 (GRIN2B) S383 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q13554-1 (CAMK2B) T287 Uniprot
Q9NQC7 (CYLD) S362 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S772 Uniprot

Research Backgrounds

Function:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

PTMs:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse.
Note: In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

Subunit Structure:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other Ref.19). Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ. Interacts with FOXO3 (By similarity). Interacts (when in a kinase inactive state not associated with calmodulin) with ARC; leading to target ARC to inactive synapses (By similarity).

Family&Domains:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

References

1). Neuroprotective Effects of Dammarane-Type Saponins from Panax notoginseng on Glutamate-Induced Cell Damage in PC12 Cells. PLANTA MEDICA, 2019 (PubMed: 30791058) [IF=2.7]

Application: WB    Species: rat    Sample: PC12 cells

Fig. 6 | Effects of compound 4 on the protein expression levels in glutamate-treated PC12 cells. A Western blot analysis of compound 4 and MK801 (a standard NMDAR antagonist) for the expression of p-NMDAR1/NMDAR1. B Western blot analysis of compound 4 and KN93 (a standard inhibitor of CaMKII) for the expression of p-CaMKII/CaMKII. Results were obtained from three independent experiments and are expressed as the mean ± SEM (##p < 0.01 vs. control group, **p < 0.01 vs. glutamate-induced cells, *p < 0.05 vs. glutamate-induced cells).

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