Phospho-Cyclin E1 (Thr395) Antibody

WB
IHC
IF/ICC
pElisa

1/4

Western blot analysis of Cyclin E1 phosphorylation expression in Paclitaxel treated HeLa whole cell lysates,The lane on the left was treated with the antigen-specific peptide.

AF3235 at 1/200 staining human Liver cancer tissue sections by IHC-P.

AF3235 staining HepG2 cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF3235.

Product:	Phospho-Cyclin E1 (Thr395) Antibody
Catalog:	AF3235
Description:	Rabbit polyclonal antibody to Phospho-Cyclin E1 (Thr395)
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	48kDa; 47kD(Calculated).
Uniprot:	P24864
RRID:	AB_2834661

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(82%), Horse(80%), Sheep(82%)

Clonality:

Polyclonal

Specificity:

Phospho-Cyclin E1 (Thr395) Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Threonine 395.

RRID:

AB_2834661
Cite Format: Affinity Biosciences Cat# AF3235, RRID:AB_2834661.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1;

Immunogens

Immunogen:

Uniprot:

P24864(CCNE1_HUMAN) >>Visit HPA database.

Gene(ID):

CCNE1(898)

Expression:

P24864 CCNE1_HUMAN:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Description:

a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases.

Sequence:

P24864 CCNE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Horse

Pig

Xenopus

Chicken

Zebrafish

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24864 As Substrate

P24864

Site	PTM Type	Enzyme	Source
S59	Phosphorylation		Uniprot
S73	Phosphorylation	P49841 (GSK3B)	Uniprot
T77	Phosphorylation	P49841 (GSK3B)	Uniprot
S90	Phosphorylation		Uniprot
S103	Phosphorylation		Uniprot
S381	Phosphorylation		Uniprot
S387	Phosphorylation	P24941 (CDK2)	Uniprot
S391	Phosphorylation		Uniprot
T395	Phosphorylation	P49841 (GSK3B) , P24941 (CDK2) , P49840 (GSK3A)	Uniprot
S399	Phosphorylation	P24941 (CDK2)	Uniprot

PTMs - P24864 As Enzyme

P24864

Substrate	Site	Source
Q8TDN4-2 (CABLES1)	S48	Uniprot

Research Backgrounds

P24864_CCNE1_HUMAN

Function:

Essential for the control of the cell cycle at the G1/S (start) transition.

PTMs:

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.

Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Subcellular Location:

Nucleus.

Tissue Specificity:

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Subunit Structure:

Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Interacts with INCA1.

Family&Domains:

Belongs to the cyclin family. Cyclin E subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

References

1). AKAP95 regulates ubiquitination and degradation of cyclin Ds/Es, influencing the G1/S transition of lung cancer cells. Molecular carcinogenesis, 2024 (PubMed: 38923703) [IF=4.6]

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Phospho-Cyclin E1 (Thr395) Antibody - #AF3235