Phospho-TYK2 (Tyr433) Antibody - #AF7288

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Product: Phospho-TYK2 (Tyr433) Antibody
Catalog: AF7288
Description: Rabbit polyclonal antibody to Phospho-TYK2 (Tyr433)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 140kDa; 134kD(Calculated).
Uniprot: P29597
RRID: AB_2843728

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 100ul $350 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-TYK2 (Tyr433) Antibody detects endogenous levels of TYK2 only when phosphorylated at Tyr433.
RRID:
AB_2843728
Cite Format: Affinity Biosciences Cat# AF7288, RRID:AB_2843728.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

JTK 1; JTK1; Non receptor tyrosine protein kinase 2; Non receptor tyrosine protein kinase TYK2; Non-receptor tyrosine-protein kinase TYK2; OTTHUMP00000232745; OTTHUMP00000232746; OTTHUMP00000232748; Protein Tyrosine Kinase 2; TYK 2; Tyk2; TYK2_HUMAN; Tyrosine kinase 2;

Immunogens

Immunogen:
Uniprot:

P29597(TYK2_HUMAN) >>Visit HPA database.

Gene(ID):
TYK2(7297)
Expression:
P29597 TYK2_HUMAN:

Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.

Sequence:
MPLRHWGMARGSKPVGDGAQPMAAMGGLKVLLHWAGPGGGEPWVTFSESSLTAEEVCIHIAHKVGITPPCFNLFALFDAQAQVWLPPNHILEIPRDASLMLYFRIRFYFRNWHGMNPREPAVYRCGPPGTEASSDQTAQGMQLLDPASFEYLFEQGKHEFVNDVASLWELSTEEEIHHFKNESLGMAFLHLCHLALRHGIPLEEVAKKTSFKDCIPRSFRRHIRQHSALTRLRLRNVFRRFLRDFQPGRLSQQMVMVKYLATLERLAPRFGTERVPVCHLRLLAQAEGEPCYIRDSGVAPTDPGPESAAGPPTHEVLVTGTGGIQWWPVEEEVNKEEGSSGSSGRNPQASLFGKKAKAHKAVGQPADRPREPLWAYFCDFRDITHVVLKEHCVSIHRQDNKCLELSLPSRAAALSFVSLVDGYFRLTADSSHYLCHEVAPPRLVMSIRDGIHGPLLEPFVQAKLRPEDGLYLIHWSTSHPYRLILTVAQRSQAPDGMQSLRLRKFPIEQQDGAFVLEGWGRSFPSVRELGAALQGCLLRAGDDCFSLRRCCLPQPGETSNLIIMRGARASPRTLNLSQLSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLADVLTVNPDSPASDPTVFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
90
Bovine
90
Sheep
90
Xenopus
90
Zebrafish
70
Rabbit
44
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29597 As Substrate

Site PTM Type Enzyme
T67 Phosphorylation
Y292 Phosphorylation
S339 Phosphorylation
S340 Phosphorylation
K354 Ubiquitination
K389 Ubiquitination
K401 Ubiquitination
S406 Phosphorylation
S415 Phosphorylation
Y423 Phosphorylation
Y433 Phosphorylation
S491 Phosphorylation
S499 Phosphorylation
K504 Ubiquitination
S577 Phosphorylation
S580 Phosphorylation
K587 Ubiquitination
S613 Phosphorylation
S720 Phosphorylation
S723 Phosphorylation
K728 Ubiquitination
K756 Ubiquitination
K823 Ubiquitination
Y827 Phosphorylation
Y851 Phosphorylation
S884 Phosphorylation
K899 Ubiquitination
K930 Ubiquitination
K933 Ubiquitination
K945 Ubiquitination
K963 Ubiquitination
K1046 Ubiquitination
Y1054 Phosphorylation P29597 (TYK2) , P23458 (JAK1)
Y1055 Phosphorylation P29597 (TYK2) , P23458 (JAK1)
K1074 Ubiquitination
K1175 Ubiquitination

PTMs - P29597 As Enzyme

Substrate Site Source
O15304 (SIVA1) Y53 Uniprot
O15304 (SIVA1) Y162 Uniprot
P17181 (IFNAR1) Y466 Uniprot
P17181 (IFNAR1) Y481 Uniprot
P29597 (TYK2) Y1054 Uniprot
P29597 (TYK2) Y1055 Uniprot
P42224-2 (STAT1) Y701 Uniprot
P51452 (DUSP3) Y138 Uniprot
P63244 (RACK1) Y194 Uniprot
Q13651 (IL10RA) Y446 Uniprot
Q13651 (IL10RA) Y496 Uniprot
Q9BZL6 (PRKD2) Y438 Uniprot

Research Backgrounds

Function:

Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.

Tissue Specificity:

Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.

Subunit Structure:

Interacts with JAKMIP1.

Family&Domains:

The FERM domain mediates interaction with JAKMIP1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

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