Phospho-LDHA (Tyr239) Antibody - #AF7173

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Product: Phospho-LDHA (Tyr239) Antibody
Catalog: AF7173
Description: Rabbit polyclonal antibody to Phospho-LDHA (Tyr239)
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: P00338
RRID: AB_2843613

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Zebrafish(90%), Bovine(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-LDHA (Tyr239) Antibody detects endogenous levels of LDHA only when phosphorylated at Tyr239.
RRID:
AB_2843613
Cite Format: Affinity Biosciences Cat# AF7173, RRID:AB_2843613.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell proliferation-inducing gene 19 protein; L lactate dehydrogenase A chain; Lactate dehydrogenase A; LDH A; LDH M; LDH muscle subunit; LDH1; LDH5; LDHA; PIG 19; PIG19; Renal carcinoma antigen NY-REN-59;

Immunogens

Immunogen:

A synthesized peptide derived from human LDHA around the phosphorylation site of Tyr239.

Uniprot:

P00338(LDHA_HUMAN) >>Visit HPA database.

Gene(ID):
LDHA(3939)
Sequence:
MATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

PTMs:

ISGylated.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the LDH/MDH superfamily. LDH family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Trophoblast-derived Lactic Acid Orchestrates Decidual Macrophage Differentiation via SRC/LDHA Signaling in Early Pregnancy. International Journal of Biological Sciences, 2022 (PubMed: 35002512) [IF=8.2]

Application: WB    Species: Mouse    Sample: macrophages

Figure 8 Exogenous LA (20 mM) regulates PBMC-derived macrophage polarization through the HIF1-α/SRC/LDHA pathway. (A) ROS fluorescence intensity (n=3). (B) NAD+/NADH ratio (n = 3). (C-L) Representative data and quantification of HIF-1α, HIF-2α, p-SRC (Y418), SRC, p-LDHA (Y10), p-LDHA (Y239), LDHA, INOS, VEGF and ARG1 protein expression in macrophages treated with or without 20mM LA in the presence or absence of PX478 under normoxia and hypoxia (n = 3-6). Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01.

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