Phospho-LDHA (Tyr239) Antibody - #AF7173

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Product: Phospho-LDHA (Tyr239) Antibody
Catalog: AF7173
Description: Rabbit polyclonal antibody to Phospho-LDHA (Tyr239)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: P00338
RRID: AB_2843613

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Zebrafish(90%), Bovine(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-LDHA (Tyr239) Antibody detects endogenous levels of LDHA only when phosphorylated at Tyr239.
RRID:
AB_2843613
Cite Format: Affinity Biosciences Cat# AF7173, RRID:AB_2843613.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell proliferation-inducing gene 19 protein; L lactate dehydrogenase A chain; Lactate dehydrogenase A; LDH A; LDH M; LDH muscle subunit; LDH1; LDH5; LDHA; PIG 19; PIG19; Renal carcinoma antigen NY-REN-59;

Immunogens

Immunogen:
Uniprot:

P00338(LDHA_HUMAN) >>Visit HPA database.

Gene(ID):
LDHA(3939)
Sequence:
MATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00338 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
K5 Acetylation
K5 Methylation
K5 Ubiquitination
Y10 Phosphorylation P11362 (FGFR1) , P04626 (ERBB2) , P12931 (SRC)
K14 Acetylation
K14 Sumoylation
K14 Ubiquitination
T18 Phosphorylation
K22 Acetylation
K57 Acetylation
K57 Ubiquitination
K59 Ubiquitination
T74 Phosphorylation
K76 Ubiquitination
K81 Acetylation
K81 Ubiquitination
Y83 Phosphorylation P11362 (FGFR1)
S89 Phosphorylation
K90 Ubiquitination
T95 Phosphorylation
K118 Acetylation
K118 Methylation
K118 Ubiquitination
K126 Acetylation
K126 Ubiquitination
Y127 Phosphorylation
S128 Phosphorylation
K132 Acetylation
K132 Ubiquitination
Y145 Phosphorylation
K155 Ubiquitination
R157 Methylation
S161 Phosphorylation
S167 Phosphorylation
R169 Methylation
Y172 Phosphorylation
S184 Phosphorylation
K212 Ubiquitination
K222 Acetylation
K222 Ubiquitination
K224 Acetylation
K224 Ubiquitination
K228 Acetylation
K228 Ubiquitination
K232 Acetylation
K232 Ubiquitination
S237 Phosphorylation
Y239 Phosphorylation
K243 Acetylation
K243 Ubiquitination
K245 Ubiquitination
T248 Phosphorylation P06493 (CDK1)
S249 Phosphorylation
R269 Methylation
K278 Acetylation
K278 Methylation
K278 Ubiquitination
K284 Ubiquitination
K305 Ubiquitination
T309 Phosphorylation
S310 Phosphorylation
K317 Ubiquitination
K318 Acetylation
K318 Ubiquitination
S319 Phosphorylation
T322 Phosphorylation
K328 Acetylation
K328 Sumoylation
K328 Ubiquitination

Research Backgrounds

PTMs:

ISGylated.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the LDH/MDH superfamily. LDH family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Trophoblast-derived Lactic Acid Orchestrates Decidual Macrophage Differentiation via SRC/LDHA Signaling in Early Pregnancy. International Journal of Biological Sciences, 2022 (PubMed: 35002512) [IF=9.2]

Application: WB    Species: Mouse    Sample: macrophages

Figure 8 Exogenous LA (20 mM) regulates PBMC-derived macrophage polarization through the HIF1-α/SRC/LDHA pathway. (A) ROS fluorescence intensity (n=3). (B) NAD+/NADH ratio (n = 3). (C-L) Representative data and quantification of HIF-1α, HIF-2α, p-SRC (Y418), SRC, p-LDHA (Y10), p-LDHA (Y239), LDHA, INOS, VEGF and ARG1 protein expression in macrophages treated with or without 20mM LA in the presence or absence of PX478 under normoxia and hypoxia (n = 3-6). Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01.

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