Phospho-PGK1 (Tyr324) Antibody - #AF8608

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Product: Phospho-PGK1 (Tyr324) Antibody
Catalog: AF8608
Description: Rabbit polyclonal antibody to Phospho-PGK1 (Tyr324)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 45kDa; 45kD(Calculated).
Uniprot: P00558
RRID: AB_2843458

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 100ul $350 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PGK1 (Tyr324) Antibody detects endogenous levels of PGK1 only when phosphorylated at Y324.
RRID:
AB_2843458
Cite Format: Affinity Biosciences Cat# AF8608, RRID:AB_2843458.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2;

Immunogens

Immunogen:
Uniprot:

P00558(PGK1_HUMAN) >>Visit HPA database.

Gene(ID):
PGK1(5230)
Expression:
P00558 PGK1_HUMAN:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

Description:
The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).
Sequence:
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
86
Zebrafish
86
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00558 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S4 Phosphorylation
K6 Acetylation
K6 Ubiquitination
K11 Acetylation
K11 Ubiquitination
K15 Ubiquitination
K17 Ubiquitination
K30 Acetylation
K30 Methylation
K30 Sumoylation
K30 Ubiquitination
T35 Phosphorylation
R39 Methylation
K41 Ubiquitination
S46 Phosphorylation
K48 Acetylation
K48 Ubiquitination
C50 S-Nitrosylation
K56 Acetylation
K56 Ubiquitination
K75 Acetylation
K75 Ubiquitination
Y76 Phosphorylation
S77 Phosphorylation
K86 Acetylation
K86 Ubiquitination
S87 Phosphorylation
K91 Acetylation
K91 Ubiquitination
K97 Acetylation
K97 Ubiquitination
C99 S-Nitrosylation
K106 Acetylation
K106 Ubiquitination
C108 S-Nitrosylation
S115 Phosphorylation
R123 Methylation
K131 Acetylation
K131 Ubiquitination
K133 Acetylation
S136 Phosphorylation
K139 Ubiquitination
K141 Acetylation
K141 Ubiquitination
K146 Acetylation
K146 Ubiquitination
S153 Phosphorylation
S155 Phosphorylation
K156 Acetylation
K156 Ubiquitination
Y161 Phosphorylation
T168 Phosphorylation
S175 Phosphorylation
K184 Ubiquitination
K191 Acetylation
K191 Methylation
K191 Ubiquitination
K192 Ubiquitination
Y196 Phosphorylation
K199 Acetylation
K199 Ubiquitination
S203 Phosphorylation
K216 Acetylation
K216 Ubiquitination
K220 Acetylation
K220 Ubiquitination
T243 Phosphorylation
S256 Phosphorylation
K264 Acetylation
K264 Ubiquitination
K267 Acetylation
K267 Ubiquitination
S271 Phosphorylation
K272 Acetylation
K272 Ubiquitination
K275 Acetylation
K279 Ubiquitination
T281 Phosphorylation
T288 Phosphorylation
K291 Acetylation
K291 Ubiquitination
K297 Ubiquitination
S305 Phosphorylation
K322 Acetylation
K322 Ubiquitination
K323 Acetylation
K323 Ubiquitination
K332 Ubiquitination
K353 Acetylation
K353 Ubiquitination
K361 Acetylation
K361 Ubiquitination
R365 Methylation
C367 S-Nitrosylation
T378 Phosphorylation
C379 S-Nitrosylation
C380 S-Nitrosylation
K382 Acetylation
K382 Ubiquitination
K388 Acetylation
K388 Ubiquitination
S399 Phosphorylation
K406 Acetylation
K406 Ubiquitination
S415 Phosphorylation

PTMs - P00558 As Enzyme

Substrate Site Source
Q15118 (PDK1) T338 Uniprot

Research Backgrounds

Function:

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the phosphoglycerate kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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