Phospho-CREB (Ser133) Antibody | Affinity Biosciences

Product:	Phospho-CREB (Ser133) Antibody
Catalog:	AF3189
Description:	Rabbit polyclonal antibody to Phospho-CREB (Ser133)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	43kDa; 37kD(Calculated).
Uniprot:	P16220
RRID:	AB_2834621

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.

RRID:

AB_2834621
Cite Format: Affinity Biosciences Cat# AF3189, RRID:AB_2834621.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Active transcription factor CREB; cAMP response element binding protein 1; cAMP response element binding protein; cAMP responsive element binding protein 1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; CREB1_HUMAN; Cyclic AMP-responsive element-binding protein 1; MGC9284; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; Transactivator protein;

Immunogens

Immunogen:

Uniprot:

P16220(CREB1_HUMAN) >>Visit HPA database.

Gene(ID):

CREB1(1385)

Description:

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.

Sequence:

P16220 CREB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16220 As Substrate

P16220

Site	PTM Type	Enzyme	Source
S40	Phosphorylation		Uniprot
S80	Phosphorylation		Uniprot
S98	Phosphorylation	Q15139 (PRKD1)	Uniprot
T100	Phosphorylation	Q13315 (ATM)	Uniprot
S108	Phosphorylation	P68400 (CSNK2A1) , P48729 (CSNK1A1)	Uniprot
S111	Phosphorylation	P68400 (CSNK2A1) , P48729 (CSNK1A1) , Q13315 (ATM)	Uniprot
S114	Phosphorylation	P48729 (CSNK1A1)	Uniprot
S117	Phosphorylation		Uniprot
T119	Phosphorylation		Uniprot
S121	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
S129	Phosphorylation	P49841 (GSK3B)	Uniprot
S133	Phosphorylation	Q16539 (MAPK14) , P31751 (AKT2) , Q02156 (PRKCE) , O43781 (DYRK3) , Q96NX5 (CAMK1G) , Q16566 (CAMK4) , P17612 (PRKACA) , Q6SA08 (TSSK4) , Q99986 (VRK1) , Q13557 (CAMK2D) , Q15418 (RPS6KA1) , O75676 (RPS6KA4) , P28482 (MAPK1) , Q14012 (CAMK1) , Q06187 (BTK) , P51812 (RPS6KA3) , Q13627 (DYRK1A) , O75582 (RPS6KA5) , P41279 (MAP3K8) , O00141 (SGK1) , P27361 (MAPK3) , P31749 (AKT1) , Q13315 (ATM) , Q15349 (RPS6KA2) , Q9Y243 (AKT3) , P49137 (MAPKAPK2) , Q15139 (PRKD1)	Uniprot
Y134	Phosphorylation		Uniprot
K136	Acetylation		Uniprot
K136	Ubiquitination		Uniprot
S142	Phosphorylation	Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)	Uniprot
S143	Phosphorylation		Uniprot
S156	Phosphorylation	P48729 (CSNK1A1)	Uniprot
T256	Phosphorylation		Uniprot
S270	Phosphorylation	P06493 (CDK1)	Uniprot
S271	Phosphorylation	Q9H2X6 (HIPK2) , P06493 (CDK1)	Uniprot
K285	Sumoylation		Uniprot
K304	Sumoylation		Uniprot
K309	Ubiquitination		Uniprot
R314	Methylation		Uniprot
K323	Ubiquitination		Uniprot
K330	Ubiquitination		Uniprot
K333	Ubiquitination		Uniprot
K339	Ubiquitination		Uniprot
S340	Phosphorylation		Uniprot

Research Backgrounds

P16220_CREB1_HUMAN

Function:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

PTMs:

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-133.

Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-133. Forms a complex with KMT2A and CREBBP.

(Microbial infection) Interacts with hepatitis B virus/HBV protein X.

(Microbial infection) Interacts with HTLV-1 protein Tax.

Family&Domains:

Belongs to the bZIP family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

References

1). Qiao YL et al. Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics. PHYTOMEDICINE 2021 Jan;81:153436. (PubMed: 33360346) [IF=7.9]

Application: WB Species: mice Sample: hippocampus

Fig. 11. Effects of URE on 5-HT1A signaling pathway. (A) Western blotting analysis of 5-HT1A, BDNF, p-CREB, CREB, p-PKA, and PKA in hippocampus. (B-E) Quantitative data of 5-HT1A (B), BDNF (C), p-CREB/CREB (D), and p-PKA/PKA (E), data represent mean ± SEM (n = 3), ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the UCMS group. (F) The agonistic effect of URE against 5-HT1A receptor.

2). Ding Q et al. The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine 2021 Sep 21;S0891-5849(21)00737-1. (PubMed: 34560247) [IF=7.4]

3). Mingqiang Wang et al. Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. Phytotherapy Research 2022 Nov 25; (PubMed: 36428266) [IF=7.2]

4). Peng Z et al. MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2021 Aug 20;224:112658. (PubMed: 34425535) [IF=6.8]

Application: WB Species: Rat Sample: NG108–15 cells

Fig. 4. mRNA and protein expression of Dusp2, p38 MAPK, CREB and JNK in NG108–15 cells treated with F, Al and FA. (A/B) The graphs show the results of the statistical analysis of Dusp2, p38 MAPK, CREB and JNK mRNA expression levels based on qRT-PCR. (C/D/E/F/G/H/I) The graphs show signaling pathway protein expression in NG108–15 cells as assessed by Western blotting analysis. a P < 0.05, compared with the control group; b P < 0.05, compared with the F group; c P < 0.05, compared with the Al group (n = 6).

5). Ping Liu et al. Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function 2022 Jul 28. (PubMed: 35899807) [IF=6.1]

6). Zhang X et al. Combined effects of di (2-ethylhexyl) phthalate and bisphenol A on thyroid hormone homeostasis in adolescent female rats. Environmental science and pollution research international 2020 Jul 17. (PubMed: 32681327) [IF=5.8]

Application: IHC Species: rat Sample: thyroid

Fig. 5| Effects of DEHP and BPA on relative gene expression in the thyroid (n = 7). a Immunohistochemistry for Esr1 in the thyroid(original magnification, × 20). Scale bar = 200 μm. b The average optical density of Esr1 in the thyroid. c The mRNA expression of Esr1.d Immunohistochemistry for phosphorylation of Creb in the thyroid(original magnification, × 20).

7). Han X et al. Metformin Modulates High Glucose-Incubated Human Umbilical Vein Endothelial Cells Proliferation and Apoptosis Through AMPK/CREB/BDNF Pathway. Frontiers in Pharmacology 2018 Nov 6;9:1266 (PubMed: 30459620) [IF=5.6]

Application: WB Species: human Sample: HUVECs

FIGURE 5 | BDNF expression was regulated by metformin through CREB activation. (A) Western blot analysis of CREB and pCREB expression in HUVECs treated with NG, HG (33.3 mmol/l), and HG + MET (0.01 mmol/l) conditions. Histograms represent the relative pCREB/CREB intensity.

8). Wu J et al. Zhile Capsule Exerts Antidepressant-Like Effects through Upregulation of the BDNF Signaling Pathway and Neuroprotection. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2019 Jan 8;20(1) (PubMed: 30625977) [IF=5.6]

Application: WB Species: rat Sample: hippocampus

Figure 6.| ZL activated cAMP-CREB-BDNF signaling. (A–C) Protein expressions of BDNF, p-CREB and CREB in the hippocampus at the end of experiment determined by western blotting.Quantified protein level was normalized to that of GAPDH using densitometry (n = 3)

9). Lin Y et al. Hypericum sampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway. Journal of Ethnopharmacology 2022 Jun 07; (PubMed: 35688258) [IF=5.4]

10). Sisi Li et al. Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY) 2020 Jun 9;12. (PubMed: 32518214) [IF=5.2]

Application: WB Species: rat Sample:

Figure 6. |Sal upregulates the FGF2-mediated cAMP/PKA/CREB signaling pathway following MCAO/R. (A–G) Representative western blot bands of cAMP, PKA, CREB, p-CREB, FGF2 and FGFR1 in each group.

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Phospho-CREB (Ser133) Antibody - #AF3189