Phospho-CREB (Ser133) Antibody - #AF3189

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Product: Phospho-CREB (Ser133) Antibody
Catalog: AF3189
Description: Rabbit polyclonal antibody to Phospho-CREB (Ser133)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43kDa; 37kD(Calculated).
Uniprot: P16220
RRID: AB_2834621

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.
RRID:
AB_2834621
Cite Format: Affinity Biosciences Cat# AF3189, RRID:AB_2834621.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Active transcription factor CREB; cAMP response element binding protein 1; cAMP response element binding protein; cAMP responsive element binding protein 1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; CREB1_HUMAN; Cyclic AMP-responsive element-binding protein 1; MGC9284; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; Transactivator protein;

Immunogens

Immunogen:
Uniprot:

P16220(CREB1_HUMAN) >>Visit HPA database.

Gene(ID):
CREB1(1385)
Description:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.
Sequence:
MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16220 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
S80 Phosphorylation
S98 Phosphorylation Q15139 (PRKD1)
T100 Phosphorylation Q13315 (ATM)
S108 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1)
S111 Phosphorylation P68400 (CSNK2A1) , P48729 (CSNK1A1) , Q13315 (ATM)
S114 Phosphorylation P48729 (CSNK1A1)
S117 Phosphorylation
T119 Phosphorylation
S121 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
S129 Phosphorylation P49841 (GSK3B)
S133 Phosphorylation Q16539 (MAPK14) , P31751 (AKT2) , Q02156 (PRKCE) , O43781 (DYRK3) , Q96NX5 (CAMK1G) , Q16566 (CAMK4) , P17612 (PRKACA) , Q6SA08 (TSSK4) , Q99986 (VRK1) , Q13557 (CAMK2D) , Q15418 (RPS6KA1) , O75676 (RPS6KA4) , P28482 (MAPK1) , Q14012 (CAMK1) , Q06187 (BTK) , P51812 (RPS6KA3) , Q13627 (DYRK1A) , O75582 (RPS6KA5) , P41279 (MAP3K8) , O00141 (SGK1) , P27361 (MAPK3) , P31749 (AKT1) , Q13315 (ATM) , Q15349 (RPS6KA2) , Q9Y243 (AKT3) , P49137 (MAPKAPK2) , Q15139 (PRKD1)
Y134 Phosphorylation
K136 Acetylation
K136 Ubiquitination
S142 Phosphorylation Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)
S143 Phosphorylation
S156 Phosphorylation P48729 (CSNK1A1)
T256 Phosphorylation
S270 Phosphorylation P06493 (CDK1)
S271 Phosphorylation Q9H2X6 (HIPK2) , P06493 (CDK1)
K285 Sumoylation
K304 Sumoylation
K309 Ubiquitination
R314 Methylation
K323 Ubiquitination
K330 Ubiquitination
K333 Ubiquitination
K339 Ubiquitination
S340 Phosphorylation

Research Backgrounds

Function:

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

PTMs:

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-133.

Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity (By similarity). Interacts (phosphorylated form) with TOX3. Interacts with ARRB1. Binds to HIPK2. Interacts with SGK1. Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-133. Forms a complex with KMT2A and CREBBP.

(Microbial infection) Interacts with hepatitis B virus/HBV protein X.

(Microbial infection) Interacts with HTLV-1 protein Tax.

Family&Domains:

Belongs to the bZIP family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

References

1). Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics. PHYTOMEDICINE, 2021 (PubMed: 33360346) [IF=7.9]

Application: WB    Species: mice    Sample: hippocampus

Fig. 11. Effects of URE on 5-HT1A signaling pathway. (A) Western blotting analysis of 5-HT1A, BDNF, p-CREB, CREB, p-PKA, and PKA in hippocampus. (B-E) Quantitative data of 5-HT1A (B), BDNF (C), p-CREB/CREB (D), and p-PKA/PKA (E), data represent mean ± SEM (n = 3), ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the UCMS group. (F) The agonistic effect of URE against 5-HT1A receptor.
2). β-asarone improves cognitive impairment and alleviates autophagy in mice with vascular dementia via the cAMP/PKA/CREB pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38039902) [IF=7.9]
3). The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine, 2021 (PubMed: 34560247) [IF=7.4]
4). Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. Phytotherapy Research, 2023 (PubMed: 36428266) [IF=7.2]
5). MiR-29b-3p aggravates NG108-15 cell apoptosis triggered by fluorine combined with aluminum. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021 (PubMed: 34425535) [IF=6.8]

Application: WB    Species: Rat    Sample: NG108–15 cells

Fig. 4. mRNA and protein expression of Dusp2, p38 MAPK, CREB and JNK in NG108–15 cells treated with F, Al and FA. (A/B) The graphs show the results of the statistical analysis of Dusp2, p38 MAPK, CREB and JNK mRNA expression levels based on qRT-PCR. (C/D/E/F/G/H/I) The graphs show signaling pathway protein expression in NG108–15 cells as assessed by Western blotting analysis. a P < 0.05, compared with the control group; b P < 0.05, compared with the F group; c P < 0.05, compared with the Al group (n = 6).
6). A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ. Journal of Ginseng Research, 2022 (PubMed: 36090685) [IF=6.3]

Application: WB    Species: Rat    Sample:

Fig. 5 Effects of fluoxetine (FLU) or PPD on the immobility time in the forced swimming test (FST) (A) and tail suspension test (TST) (B) in the corticosterone (CORT)-induced depression model. Representative Western blot images of GSK 3β, p-Ser9 GSK 3β, CREB, p-Ser133 CREB, BDNF, and β-actin expression levels (C). Western blot analysis showing relative protein levels of p-Ser133 CREB (D), p-Ser9 GSK 3β (E), and BDNF (F). ∗p < 0.05 vs. the control group, nsno statistical significance vs. the control group, #p < 0.05 vs. the CORT group.
7). Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function, 2022 (PubMed: 35899807) [IF=6.1]
8). Combined effects of di (2-ethylhexyl) phthalate and bisphenol A on thyroid hormone homeostasis in adolescent female rats. Environmental science and pollution research international, 2020 (PubMed: 32681327) [IF=5.8]

Application: IHC    Species: rat    Sample: thyroid

Fig. 5| Effects of DEHP and BPA on relative gene expression in the thyroid (n = 7). a Immunohistochemistry for Esr1 in the thyroid(original magnification, × 20). Scale bar = 200 μm. b The average optical density of Esr1 in the thyroid. c The mRNA expression of Esr1.d Immunohistochemistry for phosphorylation of Creb in the thyroid(original magnification, × 20).
9). Purple sweet potato delphinidin-3-rutin represses glioma proliferation by inducing miR-20b-5p/Atg7-dependent cytostatic autophagy. Molecular Therapy - Oncolytics, 2022 (PubMed: 36090477) [IF=5.7]

Application: WB    Species: Human    Sample: U251 and A172 cells

Figure 7 PSPD3R induces autophagy in glioma cells through the Akt/Creb signaling pathway (A) U251 and A172 cells were treated with the indicated concentrations of PSPD3R for 24 h. Atg7, phosphorylation of Creb and Akt, and LC3 expression were measured by immunoblotting. (B) U251 and A172 cells were transfected with a constitutively active Akt plasmid (CA-Akt) for 24 h and were then treated with 50 μM PSPD3R for another 24 h. Cell lysates were collected and performed for immunoblotting of Akt phosphorylation, Creb phosphorylation, and LC3 lipidation. (C) U251 and A172 cells were transfected with overexpression-Creb plasmid in the presence or absence of PSPD3R (50 μM) for 24 h, and then cell lysates were collected and performed for immunoblotting of Creb and LC3 lipidation. (D) U251 and A172 cells were transfected with Creb shRNA plasmids (sh-Creb), and then cell lysates were collected and performed for immunoblotting of Creb and LC3 lipidation. (E) U251 and A172 cells were transfected with overexpression-Creb plasmid in the presence or absence of PSPD3R (50 μM) for 24 h, and then cells stained with acridine orange. Autophagosome formation of glioma cells were observed by confocal microscope. Scale bar, 10 µm. (F) U251 and A172 cells were transfected with sh-Creb plasmid or shNC, then the expression of Atg7 mRNA was measured by quantitative real-time PCR. Data are presented as mean ± SD from three independent experiments. t test for two experimental groups comparisons
10). Metformin Modulates High Glucose-Incubated Human Umbilical Vein Endothelial Cells Proliferation and Apoptosis Through AMPK/CREB/BDNF Pathway. Frontiers in Pharmacology, 2018 (PubMed: 30459620) [IF=5.6]

Application: WB    Species: human    Sample: HUVECs

FIGURE 5 | BDNF expression was regulated by metformin through CREB activation. (A) Western blot analysis of CREB and pCREB expression in HUVECs treated with NG, HG (33.3 mmol/l), and HG + MET (0.01 mmol/l) conditions. Histograms represent the relative pCREB/CREB intensity.
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