GNL3 Antibody | Affinity Biosciences

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Western blot analysis GNL3 using LOVO whole cell lysates.

Product:	GNL3 Antibody
Catalog:	DF9566
Description:	Rabbit polyclonal antibody to GNL3
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	62 kDa; 62kD(Calculated).
Uniprot:	Q9BVP2
RRID:	AB_2842762

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GNL3 Antibody detects endogenous levels of total GNL3.

RRID:

AB_2842762
Cite Format: Affinity Biosciences Cat# DF9566, RRID:AB_2842762.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C77032; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced;

Immunogens

Immunogen:

Uniprot:

Q9BVP2(GNL3_HUMAN) >>Visit HPA database.

Gene(ID):

GNL3(26354)

Expression:

Q9BVP2 GNL3_HUMAN:

Increased levels in lung tissue in cancer patients.

Sequence:

Q9BVP2 GNL3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTVLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Zebrafish

Chicken

Xenopus

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BVP2 As Substrate

Q9BVP2

Site	PTM Type	Source
K5	Acetylation	Uniprot
K17	Acetylation	Uniprot
K46	Ubiquitination	Uniprot
S53	Phosphorylation	Uniprot
K57	Ubiquitination	Uniprot
K75	Ubiquitination	Uniprot
K79	Acetylation	Uniprot
K99	Sumoylation	Uniprot
K114	Sumoylation	Uniprot
K114	Ubiquitination	Uniprot
K135	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
S141	Phosphorylation	Uniprot
K171	Ubiquitination	Uniprot
K179	Ubiquitination	Uniprot
K185	Ubiquitination	Uniprot
Y194	Phosphorylation	Uniprot
K196	Ubiquitination	Uniprot
K197	Ubiquitination	Uniprot
K224	Methylation	Uniprot
K224	Ubiquitination	Uniprot
R230	Methylation	Uniprot
K237	Acetylation	Uniprot
K237	Ubiquitination	Uniprot
K242	Ubiquitination	Uniprot
K253	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
S273	Phosphorylation	Uniprot
K275	Sumoylation	Uniprot
K275	Ubiquitination	Uniprot
T289	Phosphorylation	Uniprot
S291	Phosphorylation	Uniprot
K299	Ubiquitination	Uniprot
S312	Phosphorylation	Uniprot
S324	Phosphorylation	Uniprot
K332	Ubiquitination	Uniprot
K352	Ubiquitination	Uniprot
T366	Phosphorylation	Uniprot
K377	Ubiquitination	Uniprot
K388	Sumoylation	Uniprot
K434	Ubiquitination	Uniprot
K444	Sumoylation	Uniprot
K444	Ubiquitination	Uniprot
S490	Phosphorylation	Uniprot
T494	Phosphorylation	Uniprot
S503	Phosphorylation	Uniprot
S517	Phosphorylation	Uniprot
S529	Phosphorylation	Uniprot
K534	Sumoylation	Uniprot
Y542	Phosphorylation	Uniprot
S545	Phosphorylation	Uniprot
Y548	Phosphorylation	Uniprot

Research Backgrounds

Q9BVP2_GNL3_HUMAN

Function:

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: Shuttles between the nucleus and nucleolus.

Tissue Specificity:

Increased levels in lung tissue in cancer patients.

Subunit Structure:

Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (By similarity).

Family&Domains:

The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Belongs to the TRAFAC class YlqF/YawG GTPase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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GNL3 Antibody - #DF9566