Phospho-Tyrosine Hydroxylase (Ser40) Antibody - #AF3114

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Product: Phospho-Tyrosine Hydroxylase (Ser40) Antibody
Catalog: AF3114
Description: Rabbit polyclonal antibody to Phospho-Tyrosine Hydroxylase (Ser40)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 60kDa; 59kD(Calculated).
Uniprot: P07101
RRID: AB_2834551

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(88%), Bovine(88%), Sheep(100%), Rabbit(88%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at Serine 40.
RRID:
AB_2834551
Cite Format: Affinity Biosciences Cat# AF3114, RRID:AB_2834551.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase;

Immunogens

Immunogen:
Uniprot:

P07101(TY3H_HUMAN) >>Visit HPA database.

Gene(ID):
TH(7054)
Expression:
P07101 TY3H_HUMAN:

Mainly expressed in the brain and adrenal glands.

Description:
Tyrosine hydroxylase (EC 1.14.16.2) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Sequence:
MPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTPRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Dog
100
Bovine
88
Xenopus
88
Zebrafish
88
Rabbit
88
Pig
0
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07101 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
S19 Phosphorylation Q8IW41 (MAPKAPK5) , P49137 (MAPKAPK2) , Q9UQM7 (CAMK2A)
S40 Phosphorylation P49137 (MAPKAPK2) , P51812 (RPS6KA3) , O75582 (RPS6KA5)
S62 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S71 Phosphorylation P49137 (MAPKAPK2) , P51812 (RPS6KA3) , P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S118 Phosphorylation
Y230 Phosphorylation
Y419 Phosphorylation
S502 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the physiology of adrenergic neurons.

Tissue Specificity:

Mainly expressed in the brain and adrenal glands.

Family&Domains:

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

Restrictive clause

 

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