Product: Phospho-Tyrosine Hydroxylase (Ser19) Antibody
Catalog: AF3112
Description: Rabbit polyclonal antibody to Phospho-Tyrosine Hydroxylase (Ser19)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 60 kDa; 59kD(Calculated).
Uniprot: P07101
RRID: AB_2834549

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Sheep(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
Phospho-Tyrosine Hydroxylase (Ser19) Antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at Serine 19.
RRID:
AB_2834549
Cite Format: Affinity Biosciences Cat# AF3112, RRID:AB_2834549.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P07101 TY3H_HUMAN:

Mainly expressed in the brain and adrenal glands.

Description:
Tyrosine hydroxylase (EC 1.14.16.2) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Sequence:
MPTPDATTPQAKGFRRAVSELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASYTPTPRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
73
Pig
0
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07101 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
S19 Phosphorylation Q8IW41 (MAPKAPK5) , P49137 (MAPKAPK2) , Q9UQM7 (CAMK2A)
S40 Phosphorylation P49137 (MAPKAPK2) , P51812 (RPS6KA3) , O75582 (RPS6KA5)
S62 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S71 Phosphorylation P49137 (MAPKAPK2) , P51812 (RPS6KA3) , P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S118 Phosphorylation
Y230 Phosphorylation
Y419 Phosphorylation
S502 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the physiology of adrenergic neurons.

Tissue Specificity:

Mainly expressed in the brain and adrenal glands.

Family&Domains:

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.