ANGPT4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of ANGPT4 using RAW264.

DF9209 at 1/100 staining rat kidney tissue by IHC-P.

Product:	ANGPT4 Antibody
Catalog:	DF9209
Description:	Rabbit polyclonal antibody to ANGPT4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	Q9Y264
RRID:	AB_2842405

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

ANGPT4 Antibody detects endogenous levels of total ANGPT4.

RRID:

AB_2842405
Cite Format: Affinity Biosciences Cat# DF9209, RRID:AB_2842405.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AGP4; ANG-3; ANG-4; ANG3; ANG4; Angiopoietin 3; Angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGP4_HUMAN; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183; OTTHUMP00000029947;

Immunogens

Immunogen:

Uniprot:

Q9Y264(ANGP4_HUMAN) >>Visit HPA database.

Gene(ID):

ANGPT4(51378)

Expression:

Q9Y264 ANGP4_HUMAN:

Highly expressed in the lung with much lower levels found in other tissues.

Sequence:

Q9Y264 ANGP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSQLAMLQGSLLLVVATMSVAQQTRQEADRGCETLVVQHGHCSYTFLLPKSEPCPPGPEVSRDSNTLQRESLANPLHLGKLPTQQVKQLEQALQNNTQWLKKLERAIKTILRSKLEQVQQQMAQNQTAPMLELGTSLLNQTTAQIRKLTDMEAQLLNQTSRMDAQMPETFLSTNKLENQLLLQRQKLQQLQGQNSALEKRLQALETKQQEELASILSKKAKLLNTLSRQSAALTNIERGLRGVRHNSSLLQDQQHSLRQLLVLLRHLVQERANASAPAFIMAGEQVFQDCAEIQRSGASASGVYTIQVSNATKPRKVFCDLQSSGGRWTLIQRRENGTVNFQRNWKDYKQGFGDPAGEHWLGNEVVHQLTRRAAYSLRVELQDWEGHEAYAQYEHFHLGSENQLYRLSVVGYSGSAGRQSSLVLQNTSFSTLDSDNDHCLCKCAQVMSGGWWFDACGLSNLNGVYYHAPDNKYKMDGIRWHYFKGPSYSLRASRMMIRPLDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Zebrafish

Chicken

Rabbit

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y264 As Substrate

Q9Y264

Site	PTM Type	Source
S72	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
T226	Phosphorylation	Uniprot
S228	Phosphorylation	Uniprot

Research Backgrounds

Q9Y264_ANGP4_HUMAN

Function:

Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.

Subcellular Location:

Secreted.

Tissue Specificity:

Highly expressed in the lung with much lower levels found in other tissues.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TEK/TIE2.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ANGPT4 Antibody - #DF9209