ALDH1A3 Antibody - #DF9187

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Product: ALDH1A3 Antibody
Catalog: DF9187
Description: Rabbit polyclonal antibody to ALDH1A3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 56 kDa; 56kD(Calculated).
Uniprot: P47895
RRID: AB_2842383

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
ALDH1A3 Antibody detects endogenous levels of total ALDH1A3.
RRID:
AB_2842383
Cite Format: Affinity Biosciences Cat# DF9187, RRID:AB_2842383.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acetaldehyde dehydrogenase 6; AL1A3_HUMAN; Aldehyde dehydrogenase 1A3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; Aldh1a3; ALDH1A6; ALDH6; RALDH 3; Retinaldehyde dehydrogenase 3; V1;

Immunogens

Immunogen:
Uniprot:

P47895(AL1A3_HUMAN) >>Visit HPA database.

Gene(ID):
ALDH1A3(220)
Expression:
P47895 AL1A3_HUMAN:

Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.

Sequence:
MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
78
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47895 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K30 Ubiquitination
K33 Ubiquitination
K44 Ubiquitination
T56 Phosphorylation
K150 Ubiquitination
K154 Ubiquitination
K252 Ubiquitination
K263 Ubiquitination
K266 Ubiquitination
T278 Phosphorylation
Y327 Phosphorylation
S328 Phosphorylation
K340 Ubiquitination
K350 Ubiquitination
K360 Ubiquitination
K364 Ubiquitination
K373 Ubiquitination
K394 Ubiquitination
K409 Ubiquitination
K421 Ubiquitination
K423 Ubiquitination
S424 Phosphorylation
K430 Ubiquitination
Y437 Phosphorylation
T440 Phosphorylation
K446 Ubiquitination
Y492 Phosphorylation
Y497 Phosphorylation
T498 Phosphorylation

Research Backgrounds

Function:

NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid. Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde. Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region (By similarity).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the aldehyde dehydrogenase family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Histidine metabolism.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

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