CDH26 Antibody | Affinity Biosciences

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CDH26 Antibody - Western blot analysis of extracts from rat brain, using CDH26 Antibody.

CDH26 Antibody - Figure 6 MAFB protected the epithelial barrier integrity of AR mouse induced by OVA.

Product:	CDH26 Antibody
Catalog:	DF8925
Description:	Rabbit polyclonal antibody to CDH26
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Sheep
Mol.Wt.:	90-100kD; 92kD(Calculated).
Uniprot:	Q8IXH8
RRID:	AB_2842121

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Sheep(86%)

Clonality:

Polyclonal

Specificity:

CDH26 Antibody detects endogenous levels of total CDH26.

RRID:

AB_2842121
Cite Format: Affinity Biosciences Cat# DF8925, RRID:AB_2842121.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CAD26_HUMAN; Cadherin 26; Cadherin like 26; Cadherin-like protein 26; Cadherin-like protein VR20; Cdh26; RP4-551D2.3; VR20;

Immunogens

Immunogen:

Uniprot:

Q8IXH8(CAD26_HUMAN) >>Visit HPA database.

Gene(ID):

CDH26(60437)

Expression:

Q8IXH8 CAD26_HUMAN:

Expressed by epithelial cells of gastrointestinal tissue.

Sequence:

Q8IXH8 CAD26_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAMRSGRHPSLLLLLVLLLWLLQVSIIDSVQQETDDLTKQTKEKIYQPLRRSKRRWVITTLELEEEDPGPFPKLIGELFNNMSYNMSLMYLISGPGVDEYPEIGLFSLEDHENGRIYVHRPVDREMTPSFTVYFDVVERSTGKIVDTSLIFNIRISDVNDHAPQFPEKEFNITVQENQSAGQPIFQMLAVDLDEENTPNSQVLYFLISQTPLLKESGFRVDRLSGEIRLSGCLDYETAPQFTLLIRARDCGEPSLSSTTTVHVDVQEGNNHRPAFTQENYKVQIPEGRASQGVLRLLVQDRDSPFTSAWRAKFNILHGNEEGHFDISTDPETNEGILNVIKPLDYETRPAQSLIIVVENEERLVFCERGKLQPPRKAAASATVSVQVTDANDPPAFHPQSFIVNKEEGARPGTLLGTFNAMDPDSQIRYELVHDPANWVSVDKNSGVVITVEPIDRESPHVNNSFYVIIIHAVDDGFPPQTATGTLMLFLSDINDNVPTLRPRSRYMEVCESAVHEPLHIEAEDPDLEPFSDPFTFELDNTWGNAEDTWKLGRNWGQSVELLTLRSLPRGNYLVPLFIGDKQGLSQKQTVHVRICPCASGLTCVELADAEVGLHVGALFPVCAAFVALAVALLFLLRCYFVLEPKRHGCSVSNDEGHQTLVMYNAESKGTSAQTWSDVEGQRPALLICTAAAGPTQGVKDLEEVPPSAASQSAQARCALGSWGYGKPFEPRSVKNIHSTPAYPDATMHRQLLAPVEGRMAETLNQKLHVANVLEDDPGYLPHVYSEEGECGGAPSLSSLASLEQELQPDLLDSLGSKATPFEEIYSESGVPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

Dog

Pig

Horse

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IXH8 As Substrate

Q8IXH8

Site	PTM Type	Source
T197	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
K405	Acetylation	Uniprot
T413	Phosphorylation	Uniprot
T417	Phosphorylation	Uniprot
S425	Phosphorylation	Uniprot

Research Backgrounds

Q8IXH8_CAD26_HUMAN

Function:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Ligand for integrins alpha-E/beta-7, ITGAE:ITGAB7, alpha-4/beta-7, ITGA4:ITGAB7 and alpha-4/beta-1, ITGA4:ITGAB1 through which modulates CD4(+) T cells activation.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed by epithelial cells of gastrointestinal tissue.

Subunit Structure:

Homodimer. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1.

Family&Domains:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

References

1). MAF bZIP Transcription Factor B (MAFB) Protected Against Ovalbumin-Induced Allergic Rhinitis via the Alleviation of Inflammation by Restoring the T Helper (Th) 1/Th2/Th17 Imbalance and Epithelial Barrier Dysfunction. Journal of Asthma and Allergy, 2022 (PubMed: 35250280) [IF=3.7]

Application: IF/ICC Species: Mouse Sample:

Figure 6 MAFB protected the epithelial barrier integrity of AR mouse induced by OVA. Mice were intraperitoneally injected with 25 μg OVA and 1 mg aluminum hydroxide gel on days 0, 7, and 14, followed by a nostril challenge with 500 μg OVA from day 21 to day 27. Selected groups of mice were injected with 1×107 TU/mL LV-MAFB into the tail vein of mice 48 h before the nostril challenge on day 19. (A) The serum content of FDA. (B) The expression of CDH26 evaluated by immunofluorescence staining (×400). Scale bar = 50 μm. (C and D) The mRNA levels of ZO-1 and occluding in the nasal mucosa of AR mice. #p<0.05, ##p<0.01.

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CDH26 Antibody - #DF8925