Phospho-c-Myc (Ser62) Antibody | Affinity Biosciences

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AF3054 at 1/100 staining mouse pancreas tissue sections by IHC-P.

AF3054 at 1/100 staining mouse spleen tissue sections by IHC-P.

AF3054 at 1/100 staining mouse testis tissue sections by IHC-P.

AF3054 at 1/100 staining human heart tissue sections by IHC-P.

AF3054 at 1/100 staining human appendiceal tissue sections by IHC-P.

AF3054 at 1/100 staining human liver tissue sections by IHC-P.

AF3054 at 1/100 staining rat uterine tissue sections by IHC-P.

AF3054 at 1/100 staining rat kidney tissue sections by IHC-P.

AF3054 at 1/100 staining rat ovarian tissue sections by IHC-P.

AF3054 staining HeLa cells treated with Forskolin 40nM 30' by ICC/IF.

peptide-ELISA analysis of AF3054.

Fig.

Product:	Phospho-c-Myc (Ser62) Antibody
Catalog:	AF3054
Description:	Rabbit polyclonal antibody to Phospho-c-Myc (Ser62)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	50~60kDa; 49kD(Calculated).
Uniprot:	P01106
RRID:	AB_2834481

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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WB Handbook

IHC Protocol

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Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-c-Myc (Ser62) Antibody detects endogenous levels of c-Myc only when phosphorylated at Serine 62.

RRID:

AB_2834481
Cite Format: Affinity Biosciences Cat# AF3054, RRID:AB_2834481.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AU016757; Avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; Class E basic helix-loop-helix protein 39; MRTL; Myc; Myc protein; Myc proto oncogene protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; MYC_HUMAN; Myc2; MYCC; Myelocytomatosis oncogene; Niard; Nird; Oncogene Myc; OTTHUMP00000158589; Proto-oncogene c-Myc; Protooncogene homologous to myelocytomatosis virus; RNCMYC; Transcription factor p64; Transcriptional regulator Myc-A; V-Myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian);

Immunogens

Immunogen:

Uniprot:

P01106(MYC_HUMAN) >>Visit HPA database.

Gene(ID):

MYC(4609)

Description:

Myc a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors.. Seems to activate the transcription of growth-related genes.

Sequence:

P01106 MYC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01106 As Substrate

P01106

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
S6	Phosphorylation		Uniprot
T8	Phosphorylation	P04049 (RAF1)	Uniprot
Y12	Phosphorylation		Uniprot
Y16	Phosphorylation		Uniprot
Y22	Phosphorylation		Uniprot
Y32	Phosphorylation	P00519 (ABL1)	Uniprot
K51	Sumoylation		Uniprot
K51	Ubiquitination		Uniprot
K52	Sumoylation		Uniprot
K52	Ubiquitination		Uniprot
T58	O-Glycosylation		Uniprot
T58	Phosphorylation	P49841 (GSK3B) , P28482 (MAPK1) , P49840 (GSK3A) , P53779 (MAPK10) , P45983 (MAPK8)	Uniprot
S62	Phosphorylation	Q13627 (DYRK1A) , P45984 (MAPK9) , Q00535 (CDK5) , P28482 (MAPK1) , P45983 (MAPK8) , Q92630 (DYRK2) , P53779 (MAPK10) , P42345 (MTOR)	Uniprot
S64	Phosphorylation		Uniprot
S67	Phosphorylation		Uniprot
S71	Phosphorylation	P28482 (MAPK1) , P45984 (MAPK9) , P53779 (MAPK10) , P45983 (MAPK8)	Uniprot
Y74	Phosphorylation	P00519 (ABL1)	Uniprot
T78	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
K143	Acetylation		Uniprot
K148	Acetylation		Uniprot
K148	Sumoylation		Uniprot
K148	Ubiquitination		Uniprot
S151	Phosphorylation		Uniprot
K157	Acetylation		Uniprot
K157	Sumoylation		Uniprot
K157	Ubiquitination		Uniprot
S161	Phosphorylation		Uniprot
T244	Phosphorylation		Uniprot
S249	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S250	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S252	Phosphorylation	P68400 (CSNK2A1) , P48729 (CSNK1A1)	Uniprot
K275	Acetylation		Uniprot
S277	Phosphorylation		Uniprot
S279	Phosphorylation		Uniprot
S281	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
K289	Ubiquitination		Uniprot
S293	Phosphorylation		Uniprot
K298	Ubiquitination		Uniprot
K317	Acetylation		Uniprot
K317	Sumoylation		Uniprot
K323	Acetylation		Uniprot
K323	Sumoylation		Uniprot
K323	Ubiquitination		Uniprot
K326	Sumoylation		Uniprot
K326	Ubiquitination		Uniprot
S329	Phosphorylation	P11309 (PIM1) , Q9P1W9 (PIM2)	Uniprot
S337	Phosphorylation		Uniprot
T343	Phosphorylation		Uniprot
S344	Phosphorylation		Uniprot
S347	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S348	Phosphorylation	P68400 (CSNK2A1)	Uniprot
T350	Phosphorylation		Uniprot
K355	Sumoylation		Uniprot
K355	Ubiquitination		Uniprot
T358	Phosphorylation	Q13177 (PAK2)	Uniprot
K371	Acetylation		Uniprot
S373	Phosphorylation	Q13177 (PAK2)	Uniprot
K389	Sumoylation		Uniprot
K389	Ubiquitination		Uniprot
K392	Sumoylation		Uniprot
K392	Ubiquitination		Uniprot
K398	Sumoylation		Uniprot
K398	Ubiquitination		Uniprot
T400	Phosphorylation	Q13177 (PAK2)	Uniprot
S405	Phosphorylation		Uniprot
K412	Sumoylation		Uniprot
K412	Ubiquitination		Uniprot
K422	Ubiquitination		Uniprot
K430	Sumoylation		Uniprot
K430	Ubiquitination		Uniprot

Research Backgrounds

P01106_MYC_HUMAN

Function:

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).

PTMs:

Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.

Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

Subcellular Location:

Nucleus>Nucleoplasm. Nucleus>Nucleolus.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2. Interacts with RIOX1. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity. Interacts with TRIM6 (By similarity). Interacts with NPM1; the binary complex is recruited to the promoter of MYC target genes and enhances their transcription. Interacts with CIP2A; leading to the stabilization of MYC.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). The Selective SIRT3 Inhibitor 3-TYP Represses Primary Myeloma Growth by Reducing c-Myc Stability. Chemical research in toxicology, 2024 (PubMed: 38815162) [IF=4.1]

2). Cholesterol regulates cell proliferation and apoptosis of colorectal cancer by modulating miR-33a-PIM3 pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019 (PubMed: 30827510) [IF=3.1]

Application: WB Species: human Sample: CRC cells

Fig. 3.| Mechanism of miR-33a-PIM3 pathway in the role of cholesterol in CRC cells. (A) Expression of PIM3 mRNA was not changed in cholesterol treatment for 48h. (B) The changes of miR-33a after cells were transfected with miR-33a mimics or inhibitor for 24h. (C) PIM3 mRNA of cells did not show obviously difference after transfected with miR-33a mimics or inhibitor for 24h (D) PIM3 was proved to be the target gene of miR-33a by dual luciferase reporter assay. (E) The difference of expression of cell cycle and apoptotic related protein in miR-33a-PIM3 pathway between CON and CH.

3). Methyl-β-cyclodextrin potentiates the BITC-induced anti-cancer effect through modulation of the Akt phosphorylation in human colorectal cancer cells. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2018 (PubMed: 30200817) [IF=1.6]

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Phospho-c-Myc (Ser62) Antibody - #AF3054