Phospho-MEKK1 (Thr1402)[Thr1400] Antibody - #DF8741

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Product: Phospho-MEKK1 (Thr1402)[Thr1400] Antibody
Catalog: DF8741
Description: Rabbit polyclonal antibody to Phospho-MEKK1 (Thr1402)[Thr1400]
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 161 kDa; 164kD(Calculated).
Uniprot: Q13233
RRID: AB_2841945

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-MEKK1 (Thr1402) Antibody detects endogenous levels of MEKK1 only when phosphorylated at Thr1400, which site historically referenced as Thr1402.
RRID:
AB_2841945
Cite Format: Affinity Biosciences Cat# DF8741, RRID:AB_2841945.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M3K1_HUMAN; MAP3K1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK 1; Mekk; MEKK1; Mitogen activated protein kinase kinase kinase 1; Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; Mitogen-activated protein kinase kinase kinase 1; SRXY6;

Immunogens

Immunogen:

A synthesized peptide derived from human MEKK1 around the phosphorylation site of Thr1402.

Uniprot:

Q13233(M3K1_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K1(4214)
Sequence:
MAAAAGNRASSSGFPGARATSPEAGGGGGALKASSAPAAAAGLLREAGSGGRERADWRRRQLRKVRSVELDQLPEQPLFLAASPPASSTSPSPEPADAAGSGTGFQPVAVPPPHGAASRGGAHLTESVAAPDSGASSPAAAEPGEKRAPAAEPSPAAAPAGREMENKETLKGLHKMDDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPVKGDGSEMNHLAAESPGEVQASAASPASKGRRSPSPGNSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCARGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTVQQQPLAGSRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGGSSGSSPSGGATSGSSQTSISGDVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLQPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESNNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEIRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTTVPHVFSKLLEMLSVSSSTHFTRMRRRLMAIADEVEIAEAIQLGVEDTLDGQQDSFLQASVPNNYLETTENSSPECTVHLEKTGKGLCATKLSASSEDISERLASISVGPSSSTTTTTTTTEQPKPMVQTKGRPHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTATDVSKHRLQGFIPCRIPSASPQTQRKFSLQFHRNCPENKDSDKLSPVFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDPSKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

PTMs - Q13233 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T20 Phosphorylation
S21 Phosphorylation
S35 Phosphorylation
S67 Phosphorylation P01116 (KRAS) , Q13153 (PAK1)
S133 Phosphorylation
S137 Phosphorylation
S154 Phosphorylation
S232 Phosphorylation
S242 Phosphorylation
S250 Phosphorylation
S252 Phosphorylation
T261 Phosphorylation
S264 Phosphorylation
S266 Phosphorylation
S275 Phosphorylation
S281 Phosphorylation
T285 Phosphorylation
S292 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S333 Phosphorylation
K391 Ubiquitination
S431 Phosphorylation
K436 Sumoylation
S507 Phosphorylation
S511 Phosphorylation
T522 Phosphorylation
S531 Phosphorylation
T542 Phosphorylation
T657 Phosphorylation
T665 Phosphorylation
T686 Phosphorylation
K690 Ubiquitination
S796 Phosphorylation
S844 Phosphorylation
T850 Phosphorylation
K919 Ubiquitination
S921 Phosphorylation
S923 Phosphorylation
S928 Phosphorylation
S970 Phosphorylation Q13546 (RIPK1)
T999 Phosphorylation
S1016 Phosphorylation
S1018 Phosphorylation
S1026 Phosphorylation
S1043 Phosphorylation
T1082 Phosphorylation
S1087 Phosphorylation
S1089 Phosphorylation
T1114 Phosphorylation
S1157 Phosphorylation
K1168 Ubiquitination
K1176 Ubiquitination
Y1338 Phosphorylation
K1371 Ubiquitination
K1398 Ubiquitination
Y1400 Phosphorylation
Y1412 Phosphorylation
S1455 Phosphorylation

PTMs - Q13233 As Enzyme

Substrate Site Source
O14733 (MAP2K7) S271 Uniprot
O14733 (MAP2K7) T275 Uniprot
P40763 (STAT3) S727 Uniprot
P45985 (MAP2K4) T261 Uniprot
P52564 (MAP2K6) T211 Uniprot
Q02750 (MAP2K1) S218 Uniprot
Q02750 (MAP2K1) S222 Uniprot
Q13158 (FADD) S194 Uniprot
Q8NHW3 (MAFA) S14 Uniprot
Q8NHW3 (MAFA) S65 Uniprot

Research Backgrounds

Function:

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

PTMs:

Autophosphorylated.

Subunit Structure:

Binds both upstream activators and downstream substrates in multimolecular complexes through its N-terminus. Oligomerizes after binding MAP2K4 or TRAF2. Interacts with AXIN1. Interacts (via the kinase catalytic domain) with STK38. Interacts with GRIPAP1.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

References

1). New findings on post-mortem chicken quality changes: The ROS-influenced MAPK-JNK signaling pathway affects chicken quality by regulating muscle cell apoptosis. Food chemistry, 2024 (PubMed: 39018616) [IF=8.8]

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