TPO Antibody | Affinity Biosciences

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FIGURE 6 | Downregulation of TNRC6C-AS1 or overexpression of TNRC6C promotes iodine uptake of TPC1 cells.

Product:	TPO Antibody
Catalog:	DF8279
Description:	Rabbit polyclonal antibody to TPO
Application:	WB
Reactivity:	Human
Prediction:	Pig, Horse, Dog
Mol.Wt.:	102 kDa; 103kD(Calculated).
Uniprot:	P07202
RRID:	AB_2841567

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TPO Antibody detects endogenous levels of total TPO.

RRID:

AB_2841567
Cite Format: Affinity Biosciences Cat# DF8279, RRID:AB_2841567.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MSA; PERT_HUMAN; TDH2A; Thyroid microsomal antigen; Thyroid peroxidase; Thyroperoxidase; TPO; TPX;

Immunogens

Immunogen:

Uniprot:

P07202(PERT_HUMAN) >>Visit HPA database.

Gene(ID):

TPO(7173)

Sequence:

P07202 PERT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALAVLSVTLVMACTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSPAQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPPKCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDHDIAFTPQSTSKAAFGGGADCQMTCENQNPCFPIQLPEEARPAAGTACLPFYRSSAACGTGDQGALFGNLSTANPRQQMNGLTSFLDASTVYGSSPALERQLRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAPAACAPEPGIPGETRGPCFLAGDGRASEVPSLTALHTLWLREHNRLAAALKALNAHWSADAVYQEARKVVGALHQIITLRDYIPRILGPEAFQQYVGPYEGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLIRGLLARPAKLQVQDQLMNEELTERLFVLSNSSTLDLASINLQRGRDHGLPGYNEWREFCGLPRLETPADLSTAIASRSVADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKALRDGDWFWWENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWRETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADGAHPPCHASARCRNTKGGFQCLCADPYELGDDGRTCVDSGRLPRVTWISMSLAALLIGGFAGLTSTVICRWTRTGTKSTLPISETGGGTPELRCGKHQAVGTSPQRAAAQDSEQESAGMEGRDTHRLPRAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Xenopus

Bovine

Sheep

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07202 As Substrate

P07202

Site	PTM Type	Source
T52	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
T57	Phosphorylation	Uniprot
S97	Phosphorylation	Uniprot
T561	Phosphorylation	Uniprot
K879	Ubiquitination	Uniprot
T891	Phosphorylation	Uniprot

Research Backgrounds

P07202_PERT_HUMAN

Function:

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

PTMs:

Glycosylated.

Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.

Cleaved in its N-terminal part.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Cell surface.

Subunit Structure:

Interacts with DUOX1, DUOX2 and CYBA.

Family&Domains:

Belongs to the peroxidase family. XPO subfamily.

Research Fields

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer. Cancer letters, 2024 (PubMed: 37993084) [IF=9.7]

2). Long Non-coding Antisense RNA TNRC6C-AS1 Is Activated in Papillary Thyroid Cancer and Promotes Cancer Progression by Suppressing TNRC6C Expression. Frontiers in Endocrinology, 2018 (PubMed: 30038597) [IF=5.2]

Application: WB Species: human Sample: TPC1 cells

FIGURE 6 | Downregulation of TNRC6C-AS1 or overexpression of TNRC6C promotes iodine uptake of TPC1 cells. (E) Overexpression of TNRC6C by transfecting of TNRC6C overexpression plasmid for 48 h showed significant increase of protein levels of NIS, TPO, TSHR, and Pendrin in TPC1 cells compared to control cells. (GAPDH was used as the internal control).

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TPO Antibody - #DF8279