CD3G Antibody - #DF8098
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8098, RRID:AB_2841444.
Fold/Unfold
CD3 gamma; CD3-GAMMA; CD3g; CD3g antigen gamma polypeptide (TiT3 complex); CD3g antigen gamma polypeptide; CD3g molecule gamma (CD3 TCR complex); CD3g molecule gamma; CD3G_HUMAN; FLJ17620; FLJ17664; FLJ79544; FLJ94613; MGC138597; T cell antigen receptor complex gamma subunit of T3; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 gamma chain; T3G;
Immunogens
- P09693 CD3G_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEQGKGLAVLILAIILLQGTLAQSIKGNHLVKVYDYQEDGSVLLTCDAEAKNITWFKDGKMIGFLTEDKKKWNLGSNAKDPRGMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLFAEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLRRN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P09693 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| N52 | N-Glycosylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| N92 | N-Glycosylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K150 | Ubiquitination | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot |
Research Backgrounds
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Cell membrane>Single-pass type I membrane protein.
The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.
A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.
Research Fields
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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