ACTN4 Antibody | Affinity Biosciences

1/4

Western blot analysis of extracts from various samples, using ACTN4 Antibody.

DF8000 at 1/100 staining Human pancreatic cancer and para-carcinoma tissue by IHC-P.

DF8000 at 1/100 staining Human pancreatic cancer by IHC-P.

Product:	ACTN4 Antibody
Catalog:	DF8000
Description:	Rabbit polyclonal antibody to ACTN4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	105 kDa; 105kD(Calculated).
Uniprot:	O43707
RRID:	AB_2841381

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(82%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

ACTN4 Antibody detects endogenous levels of total ACTN4.

RRID:

AB_2841381
Cite Format: Affinity Biosciences Cat# DF8000, RRID:AB_2841381.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

actinin 4; Actinin alpha 4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; Alpha-actinin-4; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4;

Immunogens

Immunogen:

Uniprot:

O43707(ACTN4_HUMAN) >>Visit HPA database.

Gene(ID):

ACTN4(81)

Expression:

O43707 ACTN4_HUMAN:

Widely expressed.

Sequence:

O43707 ACTN4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Horse

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43707 As Substrate

O43707

Site	PTM Type	Enzyme	Source
Y4	Phosphorylation	Q05397 (PTK2)	Uniprot
Y11	Phosphorylation		Uniprot
Y13	Phosphorylation		Uniprot
Y31	Phosphorylation	Q05397 (PTK2)	Uniprot
K50	Ubiquitination		Uniprot
K54	Ubiquitination		Uniprot
K66	Ubiquitination		Uniprot
T69	Phosphorylation		Uniprot
S92	Phosphorylation		Uniprot
K108	Ubiquitination		Uniprot
K114	Acetylation		Uniprot
S121	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
K125	Ubiquitination		Uniprot
T142	Phosphorylation		Uniprot
S159	Phosphorylation		Uniprot
T163	Phosphorylation		Uniprot
S164	Phosphorylation		Uniprot
K181	Ubiquitination		Uniprot
S191	Phosphorylation		Uniprot
Y212	Phosphorylation		Uniprot
K214	Acetylation		Uniprot
K214	Ubiquitination		Uniprot
K217	Acetylation		Uniprot
K217	Ubiquitination		Uniprot
K233	Acetylation		Uniprot
K233	Ubiquitination		Uniprot
Y234	Phosphorylation		Uniprot
K239	Ubiquitination		Uniprot
T249	Phosphorylation		Uniprot
Y260	Phosphorylation		Uniprot
S262	Phosphorylation		Uniprot
S263	Phosphorylation		Uniprot
Y265	Phosphorylation	Q05397 (PTK2)	Uniprot
S269	Phosphorylation		Uniprot
R280	Methylation		Uniprot
K283	Acetylation		Uniprot
Y298	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
R310	Methylation		Uniprot
T312	Phosphorylation		Uniprot
R319	Methylation		Uniprot
K323	Ubiquitination		Uniprot
T324	Phosphorylation		Uniprot
K331	Acetylation		Uniprot
K331	Ubiquitination		Uniprot
Y338	Phosphorylation		Uniprot
K346	Ubiquitination		Uniprot
K350	Ubiquitination		Uniprot
C351	S-Nitrosylation		Uniprot
T359	Phosphorylation		Uniprot
T362	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
K378	Ubiquitination		Uniprot
S381	Phosphorylation		Uniprot
Y397	Phosphorylation		Uniprot
K417	Acetylation		Uniprot
K417	Ubiquitination		Uniprot
K421	Ubiquitination		Uniprot
S423	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot
K432	Acetylation		Uniprot
K432	Ubiquitination		Uniprot
K437	Acetylation		Uniprot
Y441	Phosphorylation		Uniprot
T445	Phosphorylation		Uniprot
S447	Phosphorylation		Uniprot
K450	Ubiquitination		Uniprot
K455	Acetylation		Uniprot
K455	Ubiquitination		Uniprot
S461	Phosphorylation		Uniprot
R469	Methylation		Uniprot
Y485	Phosphorylation		Uniprot
Y486	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
T493	Phosphorylation		Uniprot
K497	Ubiquitination		Uniprot
C499	S-Nitrosylation		Uniprot
S507	Phosphorylation		Uniprot
T509	Phosphorylation		Uniprot
S511	Phosphorylation		Uniprot
K521	Ubiquitination		Uniprot
Y533	Phosphorylation		Uniprot
K592	Acetylation		Uniprot
K592	Ubiquitination		Uniprot
K604	Ubiquitination		Uniprot
S608	Phosphorylation		Uniprot
Y611	Phosphorylation		Uniprot
T612	Phosphorylation		Uniprot
T615	Phosphorylation		Uniprot
S621	Phosphorylation		Uniprot
K622	Acetylation		Uniprot
K622	Ubiquitination		Uniprot
K625	Acetylation		Uniprot
K625	Ubiquitination		Uniprot
K632	Ubiquitination		Uniprot
S642	Phosphorylation		Uniprot
K643	Ubiquitination		Uniprot
S656	Phosphorylation		Uniprot
T667	Phosphorylation		Uniprot
T682	Phosphorylation		Uniprot
Y693	Phosphorylation		Uniprot
S696	Phosphorylation		Uniprot
Y700	Phosphorylation		Uniprot
K701	Ubiquitination		Uniprot
Y727	Phosphorylation		Uniprot
T741	Phosphorylation		Uniprot
T746	Phosphorylation		Uniprot
T756	Phosphorylation		Uniprot
K760	Ubiquitination		Uniprot
S763	Phosphorylation		Uniprot
S773	Phosphorylation		Uniprot
K779	Acetylation		Uniprot
K779	Methylation		Uniprot
K779	Ubiquitination		Uniprot
C793	S-Nitrosylation		Uniprot
S796	Phosphorylation		Uniprot
R805	Methylation		Uniprot
T839	Phosphorylation		Uniprot
T840	Phosphorylation		Uniprot
T842	Phosphorylation		Uniprot
T844	Phosphorylation		Uniprot
K853	Acetylation		Uniprot
K859	Acetylation		Uniprot
K859	Ubiquitination		Uniprot
R868	Methylation		Uniprot
Y878	Phosphorylation		Uniprot
Y898	Phosphorylation		Uniprot
K899	Acetylation		Uniprot
Y906	Phosphorylation		Uniprot
S909	Phosphorylation		Uniprot

Research Backgrounds

O43707_ACTN4_HUMAN

Function:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.

Subcellular Location:

Nucleus. Cytoplasm. Cell junction. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).

Tissue Specificity:

Widely expressed.

Subunit Structure:

Homodimer; antiparallel (By similarity). Binds TRIM3 at the N-terminus (By similarity). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes.

Family&Domains:

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.

Belongs to the alpha-actinin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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ACTN4 Antibody - #DF8000