Presenilin 2 Antibody - #DF7809

(1)   (3)  
Product: Presenilin 2 Antibody
Catalog: DF7809
Description: Rabbit polyclonal antibody to Presenilin 2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: P49810
RRID: AB_2841261

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Presenilin 2 Antibody detects endogenous levels of total Presenilin 2.
RRID:
AB_2841261
Cite Format: Affinity Biosciences Cat# DF7809, RRID:AB_2841261.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AD3L; AD3LP; AD4; AD5; Alzheimer disease 4; CMD1V; E5-1; OTTHUMP00000035671; OTTHUMP00000035672; OTTHUMP00000228286; OTTHUMP00000228288; Presenilin 2 (Alzheimer disease 4); Presenilin 2; Presenilin-2 CTF subunit; PS-2; PS2; Psen2; PSN2_HUMAN; PSNL2; STM-2; STM2;

Immunogens

Immunogen:
Uniprot:

P49810(PSN2_HUMAN) >>Visit HPA database.

Gene(ID):
PSEN2(5664)
Expression:
P49810 PSN2_HUMAN:

Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.

Sequence:
MLTFMASDSEEEVCDERTSLMSAESPTPRSCQEGRQGPEDGENTAQWRSQENEEDGEEDPDRYVCSGVPGRPPGLEEELTLKYGAKHVIMLFVPVTLCMIVVVATIKSVRFYTEKNGQLIYTPFTEDTPSVGQRLLNSVLNTLIMISVIVVMTIFLVVLYKYRCYKFIHGWLIMSSLMLLFLFTYIYLGEVLKTYNVAMDYPTLLLTVWNFGAVGMVCIHWKGPLVLQQAYLIMISALMALVFIKYLPEWSAWVILGAISVYDLVAVLCPKGPLRMLVETAQERNEPIFPALIYSSAMVWTVGMAKLDPSSQGALQLPYDPEMEEDSYDSFGEPSYPEVFEPPLTGYPGEELEEEEERGVKLGLGDFIFYSVLVGKAAATGSGDWNTTLACFVAILIGLCLTLLLLAVFKKALPALPISITFGLIFYFSTDNLVRPFMDTLASHQLYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49810 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation P68400 (CSNK2A1)
S9 Phosphorylation P68400 (CSNK2A1)
S19 Phosphorylation
S22 Phosphorylation
S25 Phosphorylation
T27 Phosphorylation
S49 Phosphorylation
K115 Ubiquitination
Y294 Phosphorylation
K306 Acetylation
S327 Phosphorylation P48730 (CSNK1D)
S330 Phosphorylation P48730 (CSNK1D) , P68400 (CSNK2A1)
S335 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis. Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria.

PTMs:

Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.

Phosphorylated on serine residues.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.

Subunit Structure:

Interacts with DOCK3 (By similarity). Homodimer. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Interacts with HERPUD1, FLNA, FLNB and PARL.

Family&Domains:

The PAL motif is required for normal active site conformation.

Belongs to the peptidase A22A family.

Research Fields

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

References

1). Oral Treponema denticola Infection Induces Aβ 1–40 and Aβ 1–42 Accumulation in the Hippocampus of C57BL/6 Mice. Journal of Molecular Neuroscience, 2021 (PubMed: 33763842) [IF=3.1]

Application: WB    Species: mice    Sample: hippocampi

Fig. 4 T. denticola induces Aβ accumulation by up regulating BACE1  and PS1, KMI-1303 and DAPT can inhibit the efect of T. denticola on Aβ. a  RNA was extracted from mouse hippocampus, and the expression of genes related to Aβ accumulation was detected by qPCR. The values are shown as the mean±SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001, N=4 mice per group. b, c, d, e Protein expression levels of APP, BACE1, PS1 and PS2 in the hippocampus were examined by western blotting. The results of the quantitative analysis of the blots; results are presented as means±std. dev,*p<0.05, **p<0.01, ***p<0.001, N=4 mice per group. f Images represent the results of three repeated experiments. N2a cells were treated with 107  CFU  T. denticola or 1 μmol/L DAPT and 107  CFU T. denticola or 1 μmol/L KMI1303 and 107   CFU T. denticola. T. denticola -infected N2a cells were stained by an anti-Aβ1–42 protein antibody and viewed under a fuorescence microscope. g The results of IF are expressed as means±std. dev., *p<0.05, ***p<0.001

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.