CCNA2 Antibody | Affinity Biosciences

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Western blot analysis of CCNA2 using HepG2 whole lysates.

Product:	CCNA2 Antibody
Catalog:	DF7639
Description:	Rabbit polyclonal antibody to CCNA2
Application:	WB
Reactivity:	Human
Mol.Wt.:	60 kDa; 49kD(Calculated).
Uniprot:	P20248
RRID:	AB_2841120

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CCNA2 Antibody detects endogenous levels of total CCNA2.

RRID:

AB_2841120
Cite Format: Affinity Biosciences Cat# DF7639, RRID:AB_2841120.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2;

Immunogens

Immunogen:

A synthesized peptide derived from human CCNA2, corresponding to a region within C-terminal amino acids.

Uniprot:

P20248(CCNA2_HUMAN) >>Visit HPA database.

Gene(ID):

CCNA2(890)

Sequence:

P20248 CCNA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL

PTMs - P20248 As Substrate

P20248

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
K37	Ubiquitination		Uniprot
K54	Acetylation		Uniprot
K54	Ubiquitination		Uniprot
S55	Phosphorylation		Uniprot
K68	Acetylation		Uniprot
K76	Acetylation		Uniprot
K95	Acetylation		Uniprot
K95	Ubiquitination		Uniprot
K112	Acetylation		Uniprot
Y146	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot
S154	Phosphorylation	P24941 (CDK2)	Uniprot
K192	Ubiquitination		Uniprot
S209	Phosphorylation		Uniprot
Y225	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
T380	Phosphorylation		Uniprot
K388	Ubiquitination		Uniprot
K400	Ubiquitination		Uniprot
K417	Ubiquitination		Uniprot

PTMs - P20248 As Enzyme

P20248

Substrate	Site	Source
P62324 (BTG1)	T158	Uniprot

Research Backgrounds

P20248_CCNA2_HUMAN

Function:

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

PTMs:

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).

Subunit Structure:

Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes. Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2). Interacts with CDK2; the interaction increases from G1 to G2. Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm. Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with INCA1.

(Microbial infection) Interacts with human cytomegalovirus protein UL32.

Family&Domains:

Belongs to the cyclin family. Cyclin AB subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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For Research Use Only.
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CCNA2 Antibody - #DF7639