Product: IRF7 Antibody
Catalog: DF7503
Description: Rabbit polyclonal antibody to IRF7
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 50~70kD; 54kD(Calculated).
Uniprot: Q92985
RRID: AB_2841003

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(88%)
Clonality:
Polyclonal
Specificity:
IRF7 Antibody detects endogenous levels of total IRF7.
RRID:
AB_2841003
Cite Format: Affinity Biosciences Cat# DF7503, RRID:AB_2841003.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IMD39; Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF-7; IRF7; IRF7_HUMAN; IRF7A; IRF7B; IRF7C; IRF7H;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92985 IRF7_HUMAN:

Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

Sequence:
MALAPERAAPRVLFGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92985 As Substrate

Site PTM Type Enzyme
Ubiquitination
K50 Ubiquitination
S53 Phosphorylation
K61 Ubiquitination
K92 Acetylation
K92 Ubiquitination
K120 Ubiquitination
K303 Ubiquitination
K341 Ubiquitination
K375 Ubiquitination
T391 Phosphorylation
K444 Ubiquitination
K446 Ubiquitination
K452 Ubiquitination
S471 Phosphorylation Q9UHD2 (TBK1) , Q14164 (IKBKE)
S472 Phosphorylation Q9UHD2 (TBK1) , Q14164 (IKBKE)
S477 Phosphorylation Q14164 (IKBKE) , Q9UHD2 (TBK1)
S479 Phosphorylation Q14164 (IKBKE) , Q9UHD2 (TBK1)
S483 Phosphorylation
S487 Phosphorylation

Research Backgrounds

Function:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (By similarity). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.

PTMs:

Acetylation inhibits its DNA-binding ability and activity.

In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1.

TRAF6-mediated ubiquitination is required for IRF7 activation.

Sumoylated by TRIM28, which inhibits its transactivation activity.

(Microbial infection) Cleaved and inactivated by the protease 3C of enterovirus 71 allowing the virus to disrupt the host type I interferon production.

(Microbial infection) Cleaved and inactivated by the protease 3C of human enterovirus 68D (EV68) allowing the virus to disrupt the host type I interferon production.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The phosphorylated and active form accumulates selectively in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

Subunit Structure:

Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with MYD88 AND TRAF6.

(Microbial infection) Interacts with Epstein-Barr virus LF2 and LMP1.

(Microbial infection) Interacts with rotavirus A NSP1; this interaction leads to the proteasome-dependent degradation of IRF7.

(Microbial infection) Interacts with human herpes virus 8/HHV-8 proteins ORF45 and vIRF-1.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

(Microbial infection) Interacts with Seneca Valley virus protease 3C; this interaction is involved in the suppression of IRF7 expression and phosphorylation by the virus.

Family&Domains:

Belongs to the IRF family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

References

1). Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection. Chinese Journal of Natural Medicines, 2019 (PubMed: 31526500) [IF=4.6]

Application: WB    Species: mouse    Sample: lung

Fig. 7| mRNA and protein expression of the TLR7 signaling pathway in lung tissue. (a) Expression of TLR7, MyD88, TRAF6, IRF7, and NF-κB mRNA in lung tissue (n = 5). (b) Western blot of the protein expression of TLR7, MyD88, TRAF6, IRF7, P-IRF7, and IκBα in lung tissue (n = 3). (c) Quantification of TLR7, MyD88, TRAF6, P-IRF7/IRF7, and IκBα protein expression relative to β-actin. Data are presented as the means ± SD. *P < 0.05, **P < 0.01 vs the model group. #P < 0.05, ##P < 0.01 vs the control group

2). Annexin A2 (ANXA2) regulates the transcription and alternative splicing of inflammatory genes in renal tubular epithelial cells. BMC Genomics, 2022 [IF=4.4]

Application: WB    Species: Human    Sample: HK2 cells

Fig. 2ANXA2 regulated inflammatory gene mRNA and protein expression in HK2 cells. A Top ten GO biological processes terms enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. B Top ten KEGG functional pathways enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. C Top ten KEGG functional pathways enriched by downregulated DEGs in shANXA2 cells vs shCtrl cells. D Validation of mRNA expression of CCL5, IFI6, IFI44, IFITM1,and LTB by qRT-PCR assay. E Validation of mRNA expression of IRF7 and ISG15 by qRT-PCR assay. F Representative images showing protein levels of ANXA2, CCL5, IFI6, IFI44, IFITM1, LTB, IRF7 and ISG15 in LV-shANXA2 group vs LV-shCtrl group. Results are represented as mean ± SD.(n = 3,* P 

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