Phospho-eIF4E (Ser209) Antibody | Affinity Biosciences

WB
pElisa

1/2

Western blot analysis of Phospho-eIF4E (S209) using COLO205 whole cell lysates.

Product:	Phospho-eIF4E (Ser209) Antibody
Catalog:	DF2945
Description:	Rabbit polyclonal antibody to Phospho-eIF4E (Ser209)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	25kDa; 25kD(Calculated).
Uniprot:	P06730
RRID:	AB_2840929

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.

RRID:

AB_2840929
Cite Format: Affinity Biosciences Cat# DF2945, RRID:AB_2840929.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AUTS19; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; mRNA cap binding protein; mRNA cap-binding protein;

Immunogens

Immunogen:

Uniprot:

P06730(IF4E_HUMAN) >>Visit HPA database.

Gene(ID):

EIF4E(1977)

Sequence:

P06730 IF4E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06730 As Substrate

P06730

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T3	Phosphorylation		Uniprot
T9	Phosphorylation		Uniprot
T11	Phosphorylation		Uniprot
T16	Phosphorylation		Uniprot
T17	Phosphorylation		Uniprot
T22	Phosphorylation		Uniprot
S24	Phosphorylation		Uniprot
K36	Ubiquitination		Uniprot
S53	Phosphorylation		Uniprot
K54	Ubiquitination		Uniprot
S64	Phosphorylation		Uniprot
K119	Acetylation		Uniprot
K119	Ubiquitination		Uniprot
K159	Ubiquitination		Uniprot
K162	Ubiquitination		Uniprot
R173	Methylation		Uniprot
Y183	Phosphorylation		Uniprot
T203	Phosphorylation		Uniprot
K206	Ubiquitination		Uniprot
S207	Phosphorylation		Uniprot
S209	Phosphorylation	Q9HBH9 (MKNK2) , Q9BUB5 (MKNK1) , O00443 (PIK3C2A)	Uniprot
T210	Phosphorylation	O00443 (PIK3C2A)	Uniprot
T211	Phosphorylation		Uniprot

Research Backgrounds

P06730_IF4E_HUMAN

Function:

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

PTMs:

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Subcellular Location:

Cytoplasm>P-body. Cytoplasm.

Subunit Structure:

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with CLOCK (By similarity). Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1. Interacts with METTL3. Interacts with RBM24; this interaction prevents EIF4E from binding to p53/TP53 mRNA and inhibits the assembly of translation initiation complex.

(Microbial infection) Interacts with Lassa virus Z protein.

Family&Domains:

Belongs to the eukaryotic initiation factor 4E family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

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For Research Use Only.
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Phospho-eIF4E (Ser209) Antibody - #DF2945