Product: ENO1 Antibody
Catalog: DF2920
Description: Rabbit polyclonal antibody to ENO1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 47kDa; 47kD(Calculated).
Uniprot: P06733
RRID: AB_2840908

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ENO1 Antibody detects endogenous levels of total ENO1.
RRID:
AB_2840908
Cite Format: Affinity Biosciences Cat# DF2920, RRID:AB_2840908.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2 phospho D glycerate hydro lyase; 2-phospho-D-glycerate hydro-lyase; Alpha enolase; Alpha enolase like 1; Alpha-enolase; C myc promoter binding protein; C-myc promoter-binding protein; EC 4.2.1.11; eno1; ENO1L1; ENOA_HUMAN; Enolase 1 (alpha); Enolase 1 (alpha) like 1; Enolase 1; Enolase alpha; MBP 1; MBP-1; MBP1; MBPB1; MPB 1; MPB-1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; Plasminogen-binding protein; PPH; Tau crystallin;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P06733 ENOA_HUMAN:

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Sequence:
MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06733 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K5 Acetylation
K5 Ubiquitination
R9 Methylation
S14 Phosphorylation
R15 Methylation
T19 Phosphorylation
T26 Phosphorylation
S27 Phosphorylation
K28 Acetylation
K28 Ubiquitination
S37 Phosphorylation
S40 Phosphorylation
T41 Phosphorylation
Y44 Phosphorylation P12931 (SRC)
R50 Methylation
K54 Acetylation
K54 Ubiquitination
T55 Phosphorylation
Y57 Phosphorylation
K60 Acetylation
K60 Ubiquitination
S63 Phosphorylation
K64 Acetylation
K64 Ubiquitination
K71 Acetylation
K71 Ubiquitination
T72 Phosphorylation
S79 Phosphorylation
K80 Acetylation
K80 Ubiquitination
K81 Acetylation
K81 Ubiquitination
K89 Acetylation
K89 Ubiquitination
K92 Acetylation
K92 Ubiquitination
T100 Phosphorylation
K103 Acetylation
K103 Ubiquitination
K105 Acetylation
K105 Ubiquitination
S115 Phosphorylation
C119 S-Nitrosylation
K120 Ubiquitination
K126 Acetylation
K126 Ubiquitination
Y131 Phosphorylation
S141 Phosphorylation
S157 Phosphorylation
R183 Methylation
Y189 Phosphorylation
K193 Acetylation
K193 Ubiquitination
K197 Acetylation
K197 Ubiquitination
K199 Acetylation
K199 Ubiquitination
K202 Acetylation
K202 Ubiquitination
T205 Phosphorylation
K221 Acetylation
K221 Sumoylation
K221 Ubiquitination
K228 Acetylation
K228 Ubiquitination
T229 Phosphorylation
K233 Acetylation
K233 Ubiquitination
Y236 Phosphorylation
T237 Phosphorylation
K239 Acetylation
K239 Ubiquitination
S254 Phosphorylation
K256 Acetylation
K256 Methylation
K256 Sumoylation
K256 Ubiquitination
Y257 Phosphorylation
K262 Acetylation
K262 Sumoylation
K262 Ubiquitination
S263 Phosphorylation
Y270 Phosphorylation
S272 Phosphorylation
Y280 Phosphorylation
K281 Acetylation
K281 Ubiquitination
S282 Phosphorylation
K285 Acetylation
K285 Methylation
K285 Ubiquitination
Y287 Phosphorylation
S291 Phosphorylation
K306 Acetylation
S310 Phosphorylation
K326 Acetylation
K326 Ubiquitination
K330 Acetylation
K330 Ubiquitination
K335 Acetylation
K335 Ubiquitination
S336 Phosphorylation
C337 S-Nitrosylation
C339 S-Nitrosylation
K343 Acetylation
K343 Ubiquitination
T351 Phosphorylation
S353 Phosphorylation
K358 Acetylation
K358 Ubiquitination
S370 Phosphorylation
R372 Methylation
S373 Phosphorylation
C389 S-Nitrosylation
T390 Phosphorylation
K394 Ubiquitination
C399 S-Nitrosylation
K406 Acetylation
K406 Ubiquitination
R412 Methylation
S419 Phosphorylation
K420 Acetylation
K420 Ubiquitination
K422 Acetylation
K422 Ubiquitination
K434 Acetylation

Research Backgrounds

Function:

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

PTMs:

ISGylated.

Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.

Subcellular Location:

Cytoplasm. Cell membrane. Cytoplasm>Myofibril>Sarcomere>M line.
Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Subunit Structure:

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Isoform MBP-1 interacts with TRAPPC2B. Interacts with ENO4 and PGAM2 (By similarity). Interacts with CMTM6.

Family&Domains:

Belongs to the enolase family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). Novel long non‐coding RNA CYB561‐5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non‐small cell lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 35064752) [IF=5.3]

Application: WB    Species: human    Sample: H1299 cells

FIGURE 4| Lnc-CYB561-5 promotes aerobic glycolysis in vivo. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. *p < 0.05, **p < 0.01, ***p < 0.001,NS, p > 0.05 vs. the sh-Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one-way ANOVA followed by Tukey's post hoc test

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