Phospho-VHL (Ser68) Antibody | Affinity Biosciences

IF/ICC
pElisa
Cites

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AF8334 staining A549 cells(H2O2 treatment) by IF/ICC.

Figure 2 p-VHL content in ccRCC tissues and adjacent non-transformed ones.

Product:	Phospho-VHL (Ser68) Antibody
Catalog:	AF8334
Description:	Rabbit polyclonal antibody to Phospho-VHL (Ser68)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Dog
Mol.Wt.:	24 kDa; 24kD(Calculated).
Uniprot:	P40337
RRID:	AB_2840396

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

Phospho-VHL (Ser68) Antibody detects endogenous levels of VHL only when phosphorylated at Ser68.

RRID:

AB_2840396
Cite Format: Affinity Biosciences Cat# AF8334, RRID:AB_2840396.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Elongin binding protein; G7 protein; HRCA 1; HRCA1; Protein G7; pVHL; RCA 1; RCA1; VHL 1; VHL; VHL_HUMAN; VHL1; VHLH; Von Hippel Lindau disease tumor suppressor; von Hippel Lindau syndrome; von Hippel Lindau tumor suppressor; Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase; Von Hippel-Lindau disease tumor suppressor;

Immunogens

Immunogen:

Uniprot:

P40337(VHL_HUMAN) >>Visit HPA database.

Gene(ID):

VHL(7428)

Expression:

P40337 VHL_HUMAN:

Expressed in the adult and fetal brain and kidney.

Sequence:

P40337 VHL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Dog

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40337 As Substrate

P40337

Site	PTM Type	Enzyme	Source
S33	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S38	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S43	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S68	Phosphorylation	P49841 (GSK3B)	Uniprot
S72	Phosphorylation	O14965 (AURKA) , P48729 (CSNK1A1)	Uniprot
R79	Methylation		Uniprot
S80	Phosphorylation		Uniprot
T100	Phosphorylation	Q96PY6 (NEK1)	Uniprot
T105	Phosphorylation	Q96PY6 (NEK1)	Uniprot
S111	Phosphorylation	O96017 (CHEK2)	Uniprot
Y112	Phosphorylation	Q96PY6 (NEK1)	Uniprot
K159	Ubiquitination		Uniprot
S168	Phosphorylation	Q96PY6 (NEK1)	Uniprot
K171	Sumoylation		Uniprot
K171	Ubiquitination		Uniprot
Y175	Phosphorylation	Q96PY6 (NEK1)	Uniprot
S183	Phosphorylation	Q96PY6 (NEK1)	Uniprot
Y185	Phosphorylation		Uniprot
K196	Ubiquitination		Uniprot
T202	Phosphorylation	Q96PY6 (NEK1)	Uniprot

Research Backgrounds

P40337_VHL_HUMAN

Function:

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein. Nucleus.
Note: Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated. Colocalizes with ADRB2 at the cell membrane.

Cytoplasm. Nucleus.
Note: Equally distributed between the nucleus and the cytoplasm but not membrane-associated.

Tissue Specificity:

Expressed in the adult and fetal brain and kidney.

Subunit Structure:

Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome-dependent degradation of targeted proteins. Interacts with CUL2; this interaction is dependent on the integrity of the trimeric VBC complex. Interacts (via the beta domain) with HIF1A (via the NTAD domain); this interaction mediates degradation of HIF1A in normoxia and, in hypoxia, prevents ubiquitination and degradation of HIF1A by mediating hypoxia-induced translocation to the nucleus, a process which requires a hypoxia-dependent regulatory signal. Interacts with ADRB2; the interaction, in normoxia, is dependent on hydroxylation of ADRB2 and the subsequent VCB-mediated ubiquitination and degradation of ADRB2. Under hypoxia, hydroxylation, interaction with VHL, ubiquitination and subsequent degradation of ADRB2 are dramatically decreased. Interacts with RNF139, USP33 and JADE1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Isoform 1 and isoform 3 interact with LIMD1 (via LIM zinc-binding 2), AJUBA (via LIM domains) and WTIP (via LIM domains). Interacts with EPAS1. Interacts with CARD9.

Family&Domains:

The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].

Belongs to the VHL family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)

References

1). Regulation of Immunity in Clear Cell Renal Carcinoma: Role of PD-1, PD-L1, and PD-L2. CURRENT ISSUES IN MOLECULAR BIOLOGY, 2021 (PubMed: 34563045) [IF=3.1]

Application: WB Species: Human Sample: ccRCC tissues

Figure 2 p-VHL content in ccRCC tissues and adjacent non-transformed ones. Note: 1, 3—cancers, 2, 4—non-transformed tissues; p-VHL content is a key oncogenic event in ccRCC development. It promotes and triggers the main biological processes resulting in metabolism modification, immune regulation, apoptosis, and cell movement.

2). VHL, BAP1, PBRM1, SETD2 Expression in Clear Cell Renal Carcinoma, Association With PD-1, PD-L1, PD-L2 mRNA Level. , 2021

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For Research Use Only.
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Phospho-VHL (Ser68) Antibody - #AF8334