Product: Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody
Catalog: AF8176
Description: Rabbit polyclonal antibody to Phospho-p38 gamma/delta (Tyr185/Tyr182)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 43KD; 42kD(Calculated).
Uniprot: P53778 | O15264
RRID: AB_2840238

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody detects endogenous levels of p38 gamma/delta only when phosphorylated at Tyr185/182.
RRID:
AB_2840238
Cite Format: Affinity Biosciences Cat# AF8176, RRID:AB_2840238.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERK 6; ERK-6; ERK6; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK 12; Mapk12; Mitogen Activated Protein Kinase 12; Mitogen activated protein kinase p38 gamma; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase p38 gamma; MK12_HUMAN; P38 GAMMA; P38GAMMA; PRKM12; SAPK 3; SAPK-3; SAPK3; Stress Activated Protein Kinase 3; Stress-activated protein kinase 3; MAP kinase 13; MAP kinase p38 delta; MAPK 13; MAPK-13; Mapk13; MGC99536; Mitogen activated protein kinase 13; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase p38 delta; MK13_HUMAN; OTTHUMP00000016282; OTTHUMP00000016283; p38 delta; P38delta; PRKM13; SAPK 4; SAPK4; Stress activated protein kinase 4; Stress-activated protein kinase 4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P53778 MK12_HUMAN:

Highly expressed in skeletal muscle and heart.

O15264 MK13_HUMAN:

Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

Sequence:
MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL

MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGAYGSVCSAIDKRSGEKVAIKKLSRPFQSEIFAKRAYRELLLLKHMQHENVIGLLDVFTPASSLRNFYDFYLVMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNEDCELKILDFGLARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLTGKTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFTQLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQPFDDSLEHEKLTVDEWKQHIYKEIVNFSPIARKDSRRRSGMKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53778/O15264 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation P53778 (MAPK12)
Y27 Phosphorylation
S180 Phosphorylation
T183 Phosphorylation P46734 (MAP2K3) , P45985 (MAP2K4) , P52564 (MAP2K6)
Y185 Phosphorylation P46734 (MAP2K3) , P45985 (MAP2K4) , P52564 (MAP2K6)
K236 Ubiquitination
S362 Phosphorylation
Site PTM Type Enzyme
Y10 Phosphorylation
S27 Phosphorylation
S47 Phosphorylation
S143 Phosphorylation
T180 Phosphorylation P52564 (MAP2K6) , P46734 (MAP2K3)
Y182 Phosphorylation P52564 (MAP2K6) , P46734 (MAP2K3)
T185 Phosphorylation
K226 Ubiquitination
S261 Phosphorylation
T265 Phosphorylation
S350 Phosphorylation
S357 Phosphorylation
S361 Phosphorylation

PTMs - P53778/O15264 As Enzyme

Substrate Site Source
O00418 (EEF2K) S396 Uniprot
O43464 (HTRA2) S142 Uniprot
O60239-2 (SH3BP5) S194 Uniprot
O60239-2 (SH3BP5) S264 Uniprot
O60239-1 (SH3BP5) S351 Uniprot
O60239-1 (SH3BP5) S421 Uniprot
P05787 (KRT8) S74 Uniprot
P10636-8 (MAPT) T50 Uniprot
P10636-8 (MAPT) T69 Uniprot
P10636-6 (MAPT) T123 Uniprot
P10636-2 (MAPT) S144 Uniprot
P10636-6 (MAPT) T147 Uniprot
P10636-8 (MAPT) T153 Uniprot
P10636-6 (MAPT) T173 Uniprot
P10636-8 (MAPT) T181 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-2 (MAPT) S231 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636-8 (MAPT) S235 Uniprot
P10636-6 (MAPT) S262 Uniprot
P10636-8 (MAPT) S320 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P11388 (TOP2A) S1525 Uniprot
P15172 (MYOD1) S200 Uniprot
P15172 (MYOD1) S201 Uniprot
P15336 (ATF2) T69 Uniprot
P15336 (ATF2) T71 Uniprot
P15336 (ATF2) S90 Uniprot
P16949 (STMN1) S25 Uniprot
P26045 (PTPN3) S459 Uniprot
P30281 (CCND3) T283 Uniprot
P30307 (CDC25C) S207 Uniprot
P35222 (CTNNB1) S605 Uniprot
P53778-1 (MAPK12) S3 Uniprot
P78352 (DLG4) T287 Uniprot
P78352 (DLG4) S290 Uniprot
Q12959 (DLG1) S122 Uniprot
Q12959 (DLG1) S158 Uniprot
Q12959 (DLG1) T209 Uniprot
Q12959 (DLG1) S443 Uniprot
Q12959 (DLG1) Y447 Uniprot
Q13424-1 (SNTA1) S193 Uniprot
Q13424 (SNTA1) S201 Uniprot
Q14934 (NFATC4) S168 Uniprot
Q14934 (NFATC4) S170 Uniprot
Q8IW41 (MAPKAPK5) T182 Uniprot
Substrate Site Source
O00418 (EEF2K) S359 Uniprot
O00418 (EEF2K) S396 Uniprot
P04637 (TP53) S33 Uniprot
P04637 (TP53) S46 Uniprot
P05787 (KRT8) S74 Uniprot
P10636-8 (MAPT) T50 Uniprot
P10636-8 (MAPT) T69 Uniprot
P10636-8 (MAPT) T153 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636-8 (MAPT) S235 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P30281 (CCND3) T283 Uniprot
P78352 (DLG4) T287 Uniprot
Q13501 (SQSTM1) T269 Uniprot
Q13501 (SQSTM1) S272 Uniprot
Q14934 (NFATC4) S168 Uniprot
Q14934 (NFATC4) S170 Uniprot
Q15139 (PRKD1) S208 Uniprot
Q15139 (PRKD1) S249 Uniprot
Q15139 (PRKD1) S397 Uniprot
Q15139 (PRKD1) S401 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q8IW41 (MAPKAPK5) T182 Uniprot
Q8NHW3 (MAFA) T57 Uniprot
Q8NHW3 (MAFA) T134 Uniprot
Q8NHW3 (MAFA) S336 Uniprot

Research Backgrounds

Function:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

PTMs:

Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme.

Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion.
Note: Mitochondrial when associated with SH3BP5. In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in skeletal muscle and heart.

Subunit Structure:

Monomer. Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with SH3BP5. Interacts with LIN7C, SCRIB and SYNJ2BP (By similarity). Interacts with PTPN4; this interaction induces the activation of PTPN4 phosphatase activity.

Family&Domains:

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Function:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.

PTMs:

Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme. Dephosphorylated by dual specificity phosphatase DUSP1.

Tissue Specificity:

Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

Subunit Structure:

Interacts with MAPK8IP2.

Family&Domains:

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

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