Product: Phospho-MKNK2 (Thr249) Antibody
Catalog: AF8170
Description: Rabbit polyclonal antibody to Phospho-MKNK2 (Thr249)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: Q9HBH9
RRID: AB_2840232

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MKNK2 (Thr249) Antibody detects endogenous levels of MKNK2 only when phosphorylated at Thr249.
RRID:
AB_2840232
Cite Format: Affinity Biosciences Cat# AF8170, RRID:AB_2840232.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G protein coupled receptor kinase 7; GPRK7; MAP kinase interacting serine threonine kinase 2; MAP kinase interacting serine threonine kinase; MAP kinase interacting serine threonine protein kinase 2; MAP kinase signal integrating kinase 2; MAP kinase signal-integrating kinase 2; MAP kinase-interacting serine/threonine-protein kinase 2; MAPK interacting kinase 2; MAPK signal integrating kinase 2; Mitogen activated protein kinase interacting serine threonine kinase 2; Mknk2; MKNK2_HUMAN; Mnk2; Putative map kinase interacting kinase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9HBH9 MKNK2_HUMAN:

Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.

Sequence:
MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIPDAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDLTSFAAEAIAMNRQLAQHDEDLAEEEAAGQGQPVLVRATSRCLQLSPPSQSKLAQRRQRASLSSAPVVLVGDHA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBH9 As Substrate

Site PTM Type Enzyme
K5 Sumoylation
S15 Phosphorylation
S43 Phosphorylation P28482 (MAPK1)
T72 Phosphorylation
S74 Phosphorylation P28482 (MAPK1)
S76 Phosphorylation
K173 Ubiquitination
K207 Sumoylation
K207 Ubiquitination
S220 Phosphorylation P28482 (MAPK1)
K223 Ubiquitination
T244 Phosphorylation
T249 Phosphorylation
K326 Ubiquitination
K342 Ubiquitination
K347 Ubiquitination
T379 Phosphorylation P28482 (MAPK1)
K390 Ubiquitination
S437 Phosphorylation
S440 Phosphorylation P28482 (MAPK1)
S442 Phosphorylation
S452 Phosphorylation P28482 (MAPK1)

PTMs - Q9HBH9 As Enzyme

Substrate Site Source
O43597 (SPRY2) S112 Uniprot
O43597 (SPRY2) S121 Uniprot
P06730 (EIF4E) S209 Uniprot
P23246 (SFPQ) S8 Uniprot
P23246 (SFPQ) S283 Uniprot
Q15149 (PLEC) S4642 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal.

PTMs:

Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As(2)O(3) treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A.

Subcellular Location:

Nucleus>PML body.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.

Subunit Structure:

Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2; this interaction is promoted when MAPK pathways are repressed but repressed upon ERK proteins activation. Also binds to dephosphorylated MAPK3/ERK1 and MAPK1/ERK2. Isoform 1 interaction with phosphorylated MAPK3/ERK1 and MAPK1/ERK2 protects it from dephosphorylation and inactivation. Isoform 2 interacts with ESR2 and EIF4E in the nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

Restrictive clause

 

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