Phospho-MKNK2 (Thr249) Antibody - #AF8170
| Product: | Phospho-MKNK2 (Thr249) Antibody |
| Catalog: | AF8170 |
| Description: | Rabbit polyclonal antibody to Phospho-MKNK2 (Thr249) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 52 kDa; 52kD(Calculated). |
| Uniprot: | Q9HBH9 |
| RRID: | AB_2840232 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8170, RRID:AB_2840232.
Fold/Unfold
G protein coupled receptor kinase 7; GPRK7; MAP kinase interacting serine threonine kinase 2; MAP kinase interacting serine threonine kinase; MAP kinase interacting serine threonine protein kinase 2; MAP kinase signal integrating kinase 2; MAP kinase signal-integrating kinase 2; MAP kinase-interacting serine/threonine-protein kinase 2; MAPK interacting kinase 2; MAPK signal integrating kinase 2; Mitogen activated protein kinase interacting serine threonine kinase 2; Mknk2; MKNK2_HUMAN; Mnk2; Putative map kinase interacting kinase;
Immunogens
Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
- Q9HBH9 MKNK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVQKKPAELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIPDAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRNSCAKDLTSFAAEAIAMNRQLAQHDEDLAEEEAAGQGQPVLVRATSRCLQLSPPSQSKLAQRRQRASLSSAPVVLVGDHA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9HBH9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Sumoylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T72 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S76 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S220 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K223 | Ubiquitination | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T379 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K390 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S442 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | P28482 (MAPK1) | Uniprot |
PTMs - Q9HBH9 As Enzyme
Research Backgrounds
Serine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2)O(3)-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal.
Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase. Phosphorylated upon arsenic trioxide As(2)O(3) treatment. Phosphorylated by MAPK1/ERK2, MAPK11 and MAPK14. Dephosphorylated by PP2A.
Nucleus>PML body.
Cytoplasm.
Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2; this interaction is promoted when MAPK pathways are repressed but repressed upon ERK proteins activation. Also binds to dephosphorylated MAPK3/ERK1 and MAPK1/ERK2. Isoform 1 interaction with phosphorylated MAPK3/ERK1 and MAPK1/ERK2 protects it from dephosphorylation and inactivation. Isoform 2 interacts with ESR2 and EIF4E in the nucleus.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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