Phospho-STAT2 (Tyr631) Antibody | Affinity Biosciences

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Product:	Phospho-STAT2 (Tyr631) Antibody
Catalog:	AF8127
Description:	Rabbit polyclonal antibody to Phospho-STAT2 (Tyr631)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Xenopus
Mol.Wt.:	113kDa; 98kD(Calculated).
Uniprot:	P52630
RRID:	AB_2840190

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(83%), Horse(92%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

Phospho-STAT2 (Tyr631) Antibody detects endogenous levels of STAT2 only when phosphorylated at Tyr631.

RRID:

AB_2840190
Cite Format: Affinity Biosciences Cat# AF8127, RRID:AB_2840190.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF 3; ISGF3; MGC59816; P113; signal transducer and activator of transcription 2 113kD; Signal transducer and activator of transcription 2; STAT113; Stat2; STAT2_HUMAN;

Immunogens

Immunogen:

Uniprot:

P52630(STAT2_HUMAN) >>Visit HPA database.

Gene(ID):

STAT2(6773)

Sequence:

P52630 STAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYECGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVESQQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRRKEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELKGLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRLQEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKGPLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPKAPWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Xenopus

Dog

Sheep

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52630 As Substrate

P52630

Site	PTM Type	Enzyme	Source
S12	Phosphorylation		Uniprot
T136	Phosphorylation		Uniprot
K158	Acetylation		Uniprot
K158	Ubiquitination		Uniprot
K161	Ubiquitination		Uniprot
K167	Ubiquitination		Uniprot
K184	Ubiquitination		Uniprot
S187	Phosphorylation		Uniprot
K194	Ubiquitination		Uniprot
K197	Ubiquitination		Uniprot
K208	Ubiquitination		Uniprot
K218	Ubiquitination		Uniprot
K239	Ubiquitination		Uniprot
K244	Ubiquitination		Uniprot
K280	Ubiquitination		Uniprot
S283	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
T294	Phosphorylation		Uniprot
K295	Ubiquitination		Uniprot
K375	Acetylation		Uniprot
K375	Ubiquitination		Uniprot
K384	Acetylation		Uniprot
K384	Ubiquitination		Uniprot
T387	Phosphorylation		Uniprot
K443	Ubiquitination		Uniprot
K523	Ubiquitination		Uniprot
K543	Ubiquitination		Uniprot
K550	Ubiquitination		Uniprot
S595	Phosphorylation		Uniprot
T597	Phosphorylation		Uniprot
Y631	Phosphorylation		Uniprot
K681	Ubiquitination		Uniprot
Y690	Phosphorylation	P06239 (LCK)	Uniprot
S699	Phosphorylation		Uniprot
S734	Phosphorylation		Uniprot
S753	Phosphorylation		Uniprot
T800	Phosphorylation		Uniprot
Y833	Phosphorylation		Uniprot
S849	Phosphorylation		Uniprot

Research Backgrounds

P52630_STAT2_HUMAN

Function:

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.

PTMs:

Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response.

'Lys-48'-linked ubiquitination by DCST1 leads to STAT2 proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus upon activation by IFN-alpha/beta.

Subunit Structure:

Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation (By similarity). The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9; interacts with IRF9 in the cytoplasm (By similarity). Interacts with CRSP2 and CRSP6. Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with ARL2BP (By similarity). Interacts with E3 ubiquitin ligase DCST1; the interaction results in STAT2 ubiquitin-mediated proteasomal degradation.

(Microbial infection) Interacts with vaccinia virus protein C6.

(Microbial infection) Interacts with Simian virus 5 protein V.

(Microbial infection) Interacts with Rabies virus phosphoprotein.

(Microbial infection) Interacts with Human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth.

(Microbial infection) Interacts with Dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation.

Family&Domains:

Belongs to the transcription factor STAT family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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Phospho-STAT2 (Tyr631) Antibody - #AF8127