PSMD2 Antibody - #AF0707

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Product: PSMD2 Antibody
Catalog: AF0707
Description: Rabbit polyclonal antibody to PSMD2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 100kDa; 100kD(Calculated).
Uniprot: Q13200
RRID: AB_2834249

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
PSMD2 Antibody detects endogenous levels of total PSMD2.
RRID:
AB_2834249
Cite Format: Affinity Biosciences Cat# AF0707, RRID:AB_2834249.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; 55.11 protein; MGC14274; P97; Proteasome (prosome macropain) 26S subunit non ATPase 2; Proteasome 26S subunit, non-ATPase, 2; Proteasome 26S, subunit 2; Protein 55.11; Psmd2; PSMD2_HUMAN; Rpn1; S2; TNFR associated protein 2; TRAP2; Tumor necrosis factor receptor associated protein 2; Tumor necrosis factor type 1 receptor-associated protein 2;

Immunogens

Immunogen:
Uniprot:

Q13200(PSMD2_HUMAN) >>Visit HPA database.

Gene(ID):
PSMD2(5708)
Expression:
Q13200 PSMD2_HUMAN:

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

Description:
PSMD2 acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence:
MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLVERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
80
Xenopus
67
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13200 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S16 Phosphorylation
T24 Phosphorylation
K27 Ubiquitination
S29 Phosphorylation
K31 Ubiquitination
K39 Ubiquitination
K41 Ubiquitination
S46 Phosphorylation
K50 Ubiquitination
K66 Ubiquitination
R79 Methylation
K94 Ubiquitination
K97 Ubiquitination
K105 Ubiquitination
K107 Ubiquitination
Y110 Phosphorylation
K119 Ubiquitination
S134 Phosphorylation
R137 Methylation
K141 Ubiquitination
S147 Phosphorylation
Y158 Phosphorylation
K168 Ubiquitination
K178 Ubiquitination
T186 Phosphorylation
K189 Ubiquitination
Y194 Phosphorylation
Y226 Phosphorylation
K228 Ubiquitination
K286 Ubiquitination
K292 Ubiquitination
K343 Ubiquitination
Y349 Phosphorylation
K350 Acetylation
K350 Ubiquitination
R357 Methylation
S361 Phosphorylation
S363 Phosphorylation
K397 Ubiquitination
Y432 Phosphorylation
Y434 Phosphorylation
S435 Phosphorylation
S436 Phosphorylation
Y439 Phosphorylation
K441 Ubiquitination
S442 Phosphorylation
T499 Phosphorylation
S508 Phosphorylation
K551 Ubiquitination
Y554 Phosphorylation
K623 Ubiquitination
S711 Phosphorylation
K712 Ubiquitination
K754 Ubiquitination
K773 Ubiquitination
K858 Ubiquitination
K860 Ubiquitination
K902 Ubiquitination
Y906 Phosphorylation

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.

Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.

Tissue Specificity:

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD2. Interacts with RPGRIP1L (By similarity). Interacts with CRY1 in a KDM8-dependent manner (By similarity).

Family&Domains:

Belongs to the proteasome subunit S2 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

Restrictive clause

 

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