ETV4 Antibody - #AF0435
| Product: | ETV4 Antibody |
| Catalog: | AF0435 |
| Description: | Rabbit polyclonal antibody to ETV4 |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 53kDa; 54kD(Calculated). |
| Uniprot: | P43268 |
| RRID: | AB_2834238 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0435, RRID:AB_2834238.
Fold/Unfold
Adenovirus E1A enhancer binding protein; Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein E1AF); ETV4; ETV4_HUMAN; PEA3; PEA3 protein; PEAS3; Polyomavirus enhancer activator 3; Polyomavirus enhancer activator 3 homolog; Protein PEA3;
Immunogens
- P43268 ETV4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRMKAGYLDQQVPYTFSSKSPGNGSLREALIGPLGKLMDPGSLPPLDSEDLFQDLSHFQETWLAEAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43268 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S73 | Phosphorylation | Uniprot | |
| K96 | Sumoylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| K226 | Sumoylation | Uniprot | |
| K260 | Sumoylation | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| Y392 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Q15831 (STK11) | Uniprot |
| Y416 | Phosphorylation | Uniprot | |
| K441 | Sumoylation | Uniprot |
Research Backgrounds
Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
Nucleus.
Belongs to the ETS family.
Research Fields
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.