ELF1 Antibody - #AF0483

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Product: ELF1 Antibody
Catalog: AF0483
Description: Rabbit polyclonal antibody to ELF1
Application: WB IF/ICC
Reactivity: Human
Prediction: Horse
Mol.Wt.: 67kDa; 67kD(Calculated).
Uniprot: P32519
RRID: AB_2834232

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(80%)
Clonality:
Polyclonal
Specificity:
ELF1 Antibody detects endogenous levels of total ELF1.
RRID:
AB_2834232
Cite Format: Affinity Biosciences Cat# AF0483, RRID:AB_2834232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E74 like factor 1; E74-like factor 1; ELF 1; ELF1; ELF1_HUMAN; Ets domain transcription factor; ETS related transcription factor Elf 1; ETS related transcription factor Elf1; ets-related transcription factor; ETS-related transcription factor Elf-1; mElf 1; p70; Sts1; TRANSCRIPTION FACTOR ELF 1;

Immunogens

Immunogen:
Uniprot:

P32519(ELF1_HUMAN) >>Visit HPA database.

Gene(ID):
ELF1(1997)
Expression:
P32519 ELF1_HUMAN:

In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.

Description:
Elf-1 Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Belongs to the ETS family. Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes.
Sequence:
MAAVVQQNDLVFEFASNVMEDERQLGDPAIFPAVIVEHVPGADILNSYAGLACVEEPNDMITESSLDVAEEEIIDDDDDDITLTVEASCHDGDETIETIEAAEALLNMDSPGPMLDEKRINNNIFSSPEDDMVVAPVTHVSVTLDGIPEVMETQQVQEKYADSPGASSPEQPKRKKGRKTKPPRPDSPATTPNISVKKKNKDGKGNTIYLWEFLLALLQDKATCPKYIKWTQREKGIFKLVDSKAVSRLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLIYINDEDPSSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVKGGATTVLKPGNSKAAKPKDPVEVAQPSEVLRTVQPTQSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQDETLNSSVQSIRTIQAPTQVPVVVSPRNQQLHTVTLQTVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQKAGSPPSIVLGPAQVQQVLTSNVQTICNGTVSVASSPSFSATAPVVTFSPRSSQLVAHPPGTVITSVIKTQETKTLTQEVEKKESEDHLKENTEKTEQQPQPYVMVVSSSNGFTSQVAMKQNELLEPNSF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32519 As Substrate

Site PTM Type Enzyme
S110 Phosphorylation
Y160 Phosphorylation
S163 Phosphorylation
S167 Phosphorylation
S168 Phosphorylation
T180 Phosphorylation
S187 Phosphorylation
T190 Phosphorylation
T191 Phosphorylation
S195 Phosphorylation
K197 Ubiquitination
K226 Ubiquitination
K229 Ubiquitination
T231 Phosphorylation
K239 Ubiquitination
K244 Ubiquitination
K256 Ubiquitination
Y261 Phosphorylation
R269 Methylation
Y271 Phosphorylation
Y272 Phosphorylation
K279 Sumoylation
K279 Ubiquitination
K290 Ubiquitination
S332 Phosphorylation
S333 Phosphorylation
S334 Phosphorylation
K346 Acetylation
S350 Phosphorylation
S376 Phosphorylation
S432 Phosphorylation
S474 Phosphorylation
K481 Ubiquitination
T559 Phosphorylation
T566 Phosphorylation
K571 Ubiquitination
S618 Phosphorylation

Research Backgrounds

Function:

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.

Subunit Structure:

Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1.

Family&Domains:

Belongs to the ETS family.

Restrictive clause

 

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