Cytochrome P450 2J2 Antibody | Affinity Biosciences

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Western blot analysis on HT29 cell lysates using Cytochrome P450 2J2 Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0713 at 1/100 staining human kidney tissue sections by IHC-P.

Product:	Cytochrome P450 2J2 Antibody
Catalog:	AF0713
Description:	Rabbit polyclonal antibody to Cytochrome P450 2J2
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	50kDa; 58kD(Calculated).
Uniprot:	P51589
RRID:	AB_2834224

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Cytochrome P450 2J2 Antibody detects endogenous levels of total Cytochrome P450 2J2.

RRID:

AB_2834224
Cite Format: Affinity Biosciences Cat# AF0713, RRID:AB_2834224.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Arachidonic acid epoxygenase; CP2J2_HUMAN; CPJ2; CYP2J2; CYPIIJ2; Cytochrome P450 2J2; Cytochrome P450 family 2 subfamily J polypeptide 2; Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; Cytochrome P450 subfamily IIJ polypeptide 2; Flavoprotein linked monooxygenase; Microsomal monooxygenase; OTTHUMP00000010550;

Immunogens

Immunogen:

Uniprot:

P51589(CP2J2_HUMAN) >>Visit HPA database.

Gene(ID):

CYP2J2(1573)

Expression:

P51589 CP2J2_HUMAN:

Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).

Description:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue.

Sequence:

P51589 CP2J2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLAAMGSLAAALWAVVHPRTLLLGTVAFLLAADFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQLFVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRFTLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV

PTMs - P51589 As Substrate

P51589

Site	PTM Type	Source
T20	Phosphorylation	Uniprot
T25	Phosphorylation	Uniprot
T141	Phosphorylation	Uniprot
T143	Phosphorylation	Uniprot
S259	Phosphorylation	Uniprot
T274	Phosphorylation	Uniprot
Y281	Phosphorylation	Uniprot
S490	Phosphorylation	Uniprot

Research Backgrounds

P51589_CP2J2_HUMAN

Function:

A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites. In combination with 15-lipoxygenase metabolizes arachidonic acid and converts hydroperoxyicosatetraenoates (HpETEs) into hydroxy epoxy eicosatrienoates (HEETs), which are precursors of vasodilatory trihydroxyicosatrienoic acids (THETAs). This hydroperoxide isomerase activity is NADPH- and O2-independent. Catalyzes the monooxygenation of a various xenobiotics, such as danazol, amiodarone, terfenadine, astemizole, thioridazine, tamoxifen, cyclosporin A and nabumetone. Catalyzes hydroxylation of the anthelmintics albendazole and fenbendazole. Catalyzes the sulfoxidation of fenbedazole.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Tissue Specificity:

Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

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Cytochrome P450 2J2 Antibody - #AF0713