GAD1 Antibody - #AF0163

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Product: GAD1 Antibody
Catalog: AF0163
Description: Rabbit polyclonal antibody to GAD1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67kDa; 67kD(Calculated).
Uniprot: Q99259
RRID: AB_2833356

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
GAD1 Antibody detects endogenous levels of total GAD1.
RRID:
AB_2833356
Cite Format: Affinity Biosciences Cat# AF0163, RRID:AB_2833356.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; DCE1_HUMAN; EC 4.1.1.15; FLJ45882; GAD 67; GAD; GAD-67; GAD1; Glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 1; Glutamate decarboxylase 1 brain 67kD; Glutamate decarboxylase 1 brain 67kDa; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase, brain, 67-KD; OTTHUMP00000041055; SCP;

Immunogens

Immunogen:
Uniprot:

Q99259(DCE1_HUMAN) >>Visit HPA database.

Gene(ID):
GAD1(2571)
Expression:
Q99259 DCE1_HUMAN:

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

Description:
GAD67 Catalyzes the production of GABA. Belongs to the group II decarboxylase family. Homodimer. 3 isoforms of the human protein are produced by alternative splicing.
Sequence:
MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEQSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADFEAKILEAKQKGYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99259 As Substrate

Site PTM Type Enzyme
K60 Ubiquitination
K68 Ubiquitination
K74 Ubiquitination
T91 Phosphorylation P17612 (PRKACA)
Y265 Phosphorylation
S571 Phosphorylation
S578 Phosphorylation

Research Backgrounds

Function:

Catalyzes the production of GABA.

Tissue Specificity:

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the group II decarboxylase family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > GABAergic synapse.

References

1). Transplantation of NEP1-40 and NT-3 Gene-Co-Transduced Neural Stem Cells Improves Function and Neurogenesis after Spinal Cord Injury in a Rat Model. NEUROLOGY INDIA, 2022 (PubMed: 36412377) [IF=2.7]

Application: IHC    Species: Rat    Sample:

Figure 4: (a) Expression of ChAT at the SCI lesion in the six groups as determined by immunohistochemical staining (×200 original magnification). (b) OD intensity of ChAT expression in all groups. (c) Expression of GAD67 at the SCI lesion in the six groups according to immunohistochemical staining (×200 original magnification). (d) OD intensity of GAD67 expression in all groups. *P < 0.05 as compared with the selected group, **P < 0.01 as compared with the selected group

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