MED17 Antibody - #AF0440

Product: MED17 Antibody
Catalog: AF0440
Description: Rabbit polyclonal antibody to MED17
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 73kDa; 73kD(Calculated).
Uniprot: Q9NVC6
RRID: AB_2834223

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MED17 Antibody detects endogenous levels of total MED17.
RRID:
AB_2834223
Cite Format: Affinity Biosciences Cat# AF0440, RRID:AB_2834223.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

77-KD subunit; Activator recruited cofactor 77 kDa component; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; CRSP6; CRSP77; DRIP80; med17; MED17_HUMAN; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of; Thyroid hormone receptor-associated protein complex 80 kDa component; Thyroid hormone receptor-associated protein, 80-KD subunit; Transcriptional coactivator CRSP77; Trap80; VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD; Vitamin D3 receptor-interacting protein complex 80 kDa component;

Immunogens

Immunogen:
Uniprot:

Q9NVC6(MED17_HUMAN) >>Visit HPA database.

Gene(ID):
MED17(9440)
Expression:
Q9NVC6 MED17_HUMAN:

Ubiquitous.

Description:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Sequence:
MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVC6 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K17 Ubiquitination
S37 Phosphorylation
S50 Phosphorylation
S53 Phosphorylation
S55 Phosphorylation
K123 Ubiquitination
T143 Phosphorylation
K149 Ubiquitination
K162 Ubiquitination
K169 Ubiquitination
K178 Ubiquitination
S185 Phosphorylation
K202 Ubiquitination
K226 Ubiquitination
K234 Ubiquitination
K235 Ubiquitination
Y256 Phosphorylation
K263 Ubiquitination
K278 Acetylation
K278 Ubiquitination
S284 Phosphorylation
K285 Ubiquitination
S288 Phosphorylation
K294 Ubiquitination
K305 Ubiquitination
K319 Ubiquitination
K353 Acetylation
K356 Acetylation
K356 Ubiquitination
K361 Acetylation
K361 Ubiquitination
K403 Ubiquitination
S408 Phosphorylation
K415 Ubiquitination
K430 Ubiquitination
K436 Ubiquitination
K591 Ubiquitination
K606 Ubiquitination
K614 Ubiquitination
K623 Ubiquitination
K629 Ubiquitination
Y637 Phosphorylation
S647 Phosphorylation

Research Backgrounds

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with GATA1 and PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2.

Family&Domains:

Belongs to the Mediator complex subunit 17 family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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