LASS4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis on HuvEc cell lysates using LASS4 Antibody,The lane on the left was treated with the antigen-specific peptide.

AF0499 at 1/100 staining human Lymph node tissue sections by IHC-P.

AF0499 staining A549 cells by IF/ICC.

Product:	LASS4 Antibody
Catalog:	AF0499
Description:	Rabbit polyclonal antibody to LASS4
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	46kDa; 46kD(Calculated).
Uniprot:	Q9HA82
RRID:	AB_2834220

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

LASS4 Antibody detects endogenous levels of total LASS4.

RRID:

AB_2834220
Cite Format: Affinity Biosciences Cat# AF0499, RRID:AB_2834220.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ceramide synthase 4; CerS4; FLJ12089; LAG1 homolog ceramide synthase 4; LAG1 longevity assurance homolog 4; LAG1, S. cerevisiae, homolog of, 4; LASS 4; LASS4; LASS4 LAG1 homolog ceramide synthase 4; LASS4_HUMAN; TRAM homolog 1; Trh 1; Trh1;

Immunogens

Immunogen:

Uniprot:

Q9HA82(CERS4_HUMAN) >>Visit HPA database.

Gene(ID):

CERS4(79603)

Description:

Lass4 May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donnors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.

Sequence:

Q9HA82 CERS4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSSFNEWFWQDRFWLPPNVTWTELEDRDGRVYPHPQDLLAALPLALVLLAMRLAFERFIGLPLSRWLGVRDQTRRQVKPNATLEKHFLTEGHRPKEPQLSLLAAQCGLTLQQTQRWFRRRRNQDRPQLTKKFCEASWRFLFYLSSFVGGLSVLYHESWLWAPVMCWDRYPNQTLKPSLYWWYLLELGFYLSLLIRLPFDVKRKDFKEQVIHHFVAVILMTFSYSANLLRIGSLVLLLHDSSDYLLEACKMVNYMQYQQVCDALFLIFSFVFFYTRLVLFPTQILYTTYYESISNRGPFFGYYFFNGLLMLLQLLHVFWSCLILRMLYSFMKKGQMEKDIRSDVEESDSSEEAAAAQEPLQLKNGAAGGPRPAPTDGPRSRVAGRLTNRHTTAT

PTMs - Q9HA82 As Substrate

Q9HA82

Site	PTM Type	Source
S342	Phosphorylation	Uniprot
S347	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
S350	Phosphorylation	Uniprot

Research Backgrounds

Q9HA82_CERS4_HUMAN

Function:

Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor.

PTMs:

Phosphorylated at the C-terminus by CK2.

N-glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Family&Domains:

The last loop motif confers selectivity toward stearoyl-CoA (octadecanoyl-CoA; C18:0-CoA) to behenoyl-CoA (docosanoyl-CoA; C22:0-CoA) as acyl donors.

Research Fields

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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LASS4 Antibody - #AF0499