AZI2 Antibody - #AF0553

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Product: AZI2 Antibody
Catalog: AF0553
Description: Rabbit polyclonal antibody to AZI2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 48kDa; 45kD(Calculated).
Uniprot: Q9H6S1
RRID: AB_2834217

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
AZI2 Antibody detects endogenous levels of total AZI2.
RRID:
AB_2834217
Cite Format: Affinity Biosciences Cat# AF0553, RRID:AB_2834217.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 azacytidine induced protein 2; 5-azacytidine-induced protein 2; AA410145; AZ2; AZI2; AZI2_HUMAN; MGC112644; Nak associated protein 1; Nak-associated protein 1; NAP1; NF kappa B activating kinase associated protein 1; NF-kappa-B-activating kinase-associated protein 1; TILP; TILP(S);

Immunogens

Immunogen:
Uniprot:

Q9H6S1(AZI2_HUMAN) >>Visit HPA database.

Gene(ID):
AZI2(64343)
Expression:
Q9H6S1 AZI2_HUMAN:

Widely expressed. Abundant expression seen in the pancreas and testis.

Description:
AZI2 Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1. Interacts with IKBKE, TBK1 and TICAM1. 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKACAPVGCSEDLGRDSTKLHLMNFTATYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGRNSLEDNSWVFPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Dog
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H6S1 As Substrate

Site PTM Type Enzyme
S83 Phosphorylation
K91 Ubiquitination
S118 Phosphorylation
S120 Phosphorylation
K122 Ubiquitination
K131 Ubiquitination
T140 Phosphorylation
T144 Phosphorylation
S155 Phosphorylation
S156 Phosphorylation
K162 Ubiquitination
S164 Phosphorylation
K192 Ubiquitination
K194 Ubiquitination
K205 Ubiquitination
S213 Phosphorylation
T249 Phosphorylation
S250 Phosphorylation
T251 Phosphorylation
K255 Ubiquitination
S263 Phosphorylation
S270 Phosphorylation
K293 Ubiquitination
S300 Phosphorylation
S301 Phosphorylation
K313 Ubiquitination
S318 Phosphorylation
S325 Phosphorylation
S338 Phosphorylation
S343 Phosphorylation
S348 Phosphorylation
S353 Phosphorylation
K356 Ubiquitination
S357 Phosphorylation

Research Backgrounds

Function:

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Abundant expression seen in the pancreas and testis.

Subunit Structure:

Homodimer (By similarity). Interacts with IKBKE. Interacts with TBK1. Interacts with TICAM1.

(Microbial infection) Interacts with vaccinia virus protein C6.

Research Fields

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

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