FIR Antibody - #AF0527

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Product: FIR Antibody
Catalog: AF0527
Description: Rabbit polyclonal antibody to FIR
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 119kDa; 120kD(Calculated).
Uniprot: O94887
RRID: AB_2834192

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(82%), Sheep(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
FIR Antibody detects endogenous levels of total FIR.
RRID:
AB_2834192
Cite Format: Affinity Biosciences Cat# AF0527, RRID:AB_2834192.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FARP2; FARP2_HUMAN; FERM; FERM domain including RhoGEF; FERM, RhoGEF and pleckstrin domain protein 2; FERM, RhoGEF and pleckstrin domain-containing protein 2; FGD1 related Cdc42 GEF; FIR; FRG; KIAA0793; PH domain-containing family C member 3; Pleckstrin homology domain-containing family C member 3; PLEKHC3; RhoGEF and pleckstrin domain protein 2; RhoGEF and pleckstrin domain-containing protein 2;

Immunogens

Immunogen:
Uniprot:

O94887(FARP2_HUMAN) >>Visit HPA database.

Gene(ID):
FARP2(9855)
Description:
Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor.Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
MGEIEGTYRVLQTAGMRLGAQTPVGVSTLEPGQTLLPRMQEKHLHLRVKLLDNTMEIFDIEPKCDGQVLLTQVWKRLNLVECDYFGMEFQNTQSYWIWLEPMKPIIRQIRRPKNVVLRLAVKFFPPDPGQLQEEYTRYLFALQLKRDLLEERLTCADTTAALLTSHLLQSEIGDYDETLDREHLKVNEYLPGQQHCLEKILEFHQKHVGQTPAESDFQVLEIARKLEMYGIRFHMASDREGTKIQLAVSHMGVLVFQGTTKINTFNWSKVRKLSFKRKRFLIKLHPEVHGPYQDTLEFLLGSRDECKNFWKICVEYHTFFRLLDQPKPKAKAVFFSRGSSFRYSGRTQKQLVDYFKDSGMKRIPYERRHSKTHTSVRALTADLPKQSISFPEGLRTPASPSSANAFYSLSPSTLVPSGLPEFKDSSSSLTDPQVSYVKSPAAERRSGAVAGGPDTPSAQPLGPPALQPGPGLSTKSPQPSPSSRKSPLSLSPAFQVPLGPAEQGSSPLLSPVLSDAGGAGMDCEEPRHKRVPADEAYFIVKEILATERTYLKDLEVITVWFRSAVVKEDAMPATLMTLLFSNIDPIYEFHRGFLREVEQRLALWEGPSKAHTKGSHQRIGDILLRNMRQLKEFTSYFQRHDEVLTELEKATKRCKKLEAVYKEFELQKVCYLPLNTFLLKPIQRLLHYRLLLRRLCGHYSPGHHDYADCHDALKAITEVTTTLQHILIRLENLQKLTELQRDLVGIENLIAPGREFIREGCLHKLTKKGLQQRMFFLFSDMLLYTSKGVAGTSHFRIRGLLPLQGMLVEESDNEWSVPHCFTIYAAQKTIVVAASTRLEKEKWMLDLNSAIQAAKSGGDTAPALPGRTVCTRPPRSPNEVSLEQESEDDARGVRSSLEGHGQHRANTTMHVCWYRNTSVSRADHSAAVENQLSGYLLRKFKNSHGWQKLWVVFTNFCLFFYKTHQDDYPLASLPLLGYSVSIPREADGIHKDYVFKLQFKSHVYFFRAESKYTFERWMEVIQGASSSAGRAPSIVQDGPQPSSGLEGMVRGKEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
82
Sheep
82
Dog
82
Chicken
73
Rabbit
64
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94887 As Substrate

Site PTM Type Enzyme
Y8 Phosphorylation
T22 Phosphorylation
S27 Phosphorylation
T28 Phosphorylation
T34 Phosphorylation
K49 Ubiquitination
K145 Ubiquitination
K185 Ubiquitination
K199 Ubiquitination
K206 Ubiquitination
K331 Ubiquitination
S336 Phosphorylation
R337 Methylation
S340 Phosphorylation
R342 Methylation
S344 Phosphorylation
K349 Ubiquitination
K356 Ubiquitination
S358 Phosphorylation
Y365 Phosphorylation
T380 Phosphorylation
K385 Ubiquitination
S387 Phosphorylation
S389 Phosphorylation
T396 Phosphorylation
S399 Phosphorylation
S401 Phosphorylation
S402 Phosphorylation
Y407 Phosphorylation
S410 Phosphorylation
T413 Phosphorylation
S425 Phosphorylation
S428 Phosphorylation
T430 Phosphorylation
S435 Phosphorylation
Y436 Phosphorylation
K438 Ubiquitination
S439 Phosphorylation
S446 Phosphorylation
S473 Phosphorylation
T474 Phosphorylation
S476 Phosphorylation
S480 Phosphorylation
Y537 Phosphorylation
T574 Phosphorylation
T577 Phosphorylation
S581 Phosphorylation
T645 Phosphorylation
T868 Phosphorylation
S876 Phosphorylation
S886 Phosphorylation
T907 Phosphorylation
T908 Phosphorylation
Y914 Phosphorylation
S1033 Phosphorylation

Research Backgrounds

Function:

Functions as guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion (By similarity).

Subunit Structure:

Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. Interacts with RAC1 (By similarity).

Family&Domains:

Intramolecular interaction between the DH domain and the PH domains can stabilize the protein in an autoinhibited conformation.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

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