TNFSF14 Antibody - #AF0329

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Product: TNFSF14 Antibody
Catalog: AF0329
Description: Rabbit polyclonal antibody to TNFSF14
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 26kDa; 26kD(Calculated).
Uniprot: O43557
RRID: AB_2834173

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
TNFSF14 Antibody detects endogenous levels of total TNFSF14.
RRID:
AB_2834173
Cite Format: Affinity Biosciences Cat# AF0329, RRID:AB_2834173.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 258; CD258; CD258 antigen; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14; TNF14_HUMAN; TNFSF 14; Tnfsf14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor (ligand) superfamily, member 14; Tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily, member 14; Tumor necrosis factor receptor like 2; tumor necrosis factor receptor-like 2; Tumor necrosis factor superfamily member 14; Tumor necrosis factor superfamily member LIGHT;

Immunogens

Immunogen:
Uniprot:

O43557(TNF14_HUMAN) >>Visit HPA database.

Gene(ID):
TNFSF14(8740)
Expression:
O43557 TNF14_HUMAN:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

Description:
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
Sequence:
MEESVVRPSVFVVDGQTDIPFTRLGRSHRRQSCSVARVGLGLLLLLMGAGLAVQGWFLLQLHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Rabbit
70
Horse
60
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43557 As Substrate

Site PTM Type Enzyme
S32 Phosphorylation
S34 Phosphorylation
T161 Phosphorylation
T163 Phosphorylation
Y167 Phosphorylation
T170 Phosphorylation

Research Backgrounds

Function:

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.

PTMs:

N-glycosylated.

The soluble form of isoform 1 derives from the membrane form by proteolytic processing.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein.

Secreted.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

Subunit Structure:

Homotrimer. Interacts with TNFRSF14.

Family&Domains:

Belongs to the tumor necrosis factor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

References

1). Hepatic stellate cell mediates transcription of TNFSF14 in hepatocellular carcinoma cells via H2S/CSE-JNK/JunB signaling pathway. Cell Death & Disease, 2022 (PubMed: 35292636) [IF=9.0]

Application: WB    Species: Human    Sample: HCC cells

Fig. 3 H2S upregulates pro-apoptotic factor TNFSF14 expression in HCC cells. A The heatmap. B Venn diagram of gene predictions based on the control group and HepG2 cells treated with NaHS and co-cultured with LX-2 cells. C The expression levels of TNFSF14 mRNA changed in HepG2 cells treated with NaHS and co-cultured with LX-2 cells. D RT-qPCR and (E) Western blotting were performed to examine the effects of various concentrations of NaHS on expression of TNFSF14 gene in HepG2 and PLC/PRF/5 cells. F RT-qPCR and (G) Western blotting analysis of the expression of TNFSF14 under 10−3 M NaHS treatment with different time in HepG2 and PLC/PRF/5 cells. H RT-qPCR and (I) Western blotting were used to detect the effects of PPG treated on TNFSF14 expression in HCC cells. All Bars, ± SD; n = 3; *P < 0.05 compared with control in HepG2 cells, #P < 0.05 compared with control in PLC/PRF/5 cells, &P < 0.05 compared with the co-culture group in HCC cells.

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