TGM2 Antibody - #AF0548

(3)  
Product: TGM2 Antibody
Catalog: AF0548
Description: Rabbit polyclonal antibody to TGM2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 77kDa; 77kD(Calculated).
Uniprot: P21980
RRID: AB_2834161

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(85%), Sheep(85%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
TGM2 Antibody detects endogenous levels of total TGM2.
RRID:
AB_2834161
Cite Format: Affinity Biosciences Cat# AF0548, RRID:AB_2834161.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALPHA SUBUNIT; C polypeptide; EC 2.3.2.13; epididymis secretory protein Li 45; G alpha h; G[a]h; Gh CLASS G ALPHA h; GNAH; GNAH G PROTEIN; H POLYPEPTIDE; HEL-S-45; Protein glutamine gamma glutamyltransferase 2; Protein-glutamine gamma-glutamyltransferase 2; TG 2; TG(C); TG2; TGase C; TGase H; TGase-2; TgaseII; TGC; TGC GUANINE NUCLEOTIDE BINDING PROTEIN; TGM2; TGM2_HUMAN; Tissue transglutaminase; Transglutaminase 2; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase-2; tTG; tTGas;

Immunogens

Immunogen:
Uniprot:

P21980(TGM2_HUMAN) >>Visit HPA database.

Gene(ID):
TGM2(7052)
Description:
TGM2 an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation.
Sequence:
MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQPGYEGWQALDPTPQEKSEGTYCCGPVPVRAIKEGDLSTKYDAPFVFAEVNADVVDWIQQDDGSVHKSINRSLIVGLKISTKSVGRDEREDITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTAEEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSEKSVPLCILYEKYRDCLTESNLIKVRALLVEPVINSYLLAERDLYLENPEIKIRILGEPKQKRKLVAEVSLQNPLPVALEGCTFTVEGAGLTEEQKTVEIPDPVEAGEEVKVRMDLLPLHMGLHKLVVNFESDKLKAVKGFRNVIIGPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Dog
92
Rabbit
92
Horse
85
Sheep
85
Chicken
58
Xenopus
46
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21980 As Substrate

Site PTM Type Enzyme
A2 Acetylation
C10 S-Nitrosylation
C27 S-Nitrosylation
S56 Phosphorylation
S60 Phosphorylation
C98 S-Nitrosylation
S129 Phosphorylation
C143 S-Nitrosylation
Y159 Phosphorylation
K202 Acetylation
K205 Acetylation
S212 Phosphorylation
S215 Phosphorylation P17612 (PRKACA)
S216 Phosphorylation P17612 (PRKACA)
Y219 Phosphorylation
C230 S-Nitrosylation
Y245 Phosphorylation
S250 Phosphorylation
C269 S-Nitrosylation
C277 S-Nitrosylation
C285 S-Nitrosylation
Y315 Phosphorylation
C336 S-Nitrosylation
T368 Phosphorylation
Y369 Phosphorylation
C370 S-Nitrosylation
C371 S-Nitrosylation
S415 Phosphorylation
S419 Phosphorylation
S427 Phosphorylation
K464 Ubiquitination
C524 S-Nitrosylation
S538 Phosphorylation
S541 Phosphorylation
C545 S-Nitrosylation
C554 S-Nitrosylation
Y575 Phosphorylation
Y583 Phosphorylation
K590 Ubiquitination
S608 Phosphorylation
C620 S-Nitrosylation
K649 Ubiquitination
K672 Acetylation

PTMs - P21980 As Enzyme

Substrate Site Source
P68431 (HIST1H3J) S11 Uniprot

Research Backgrounds

Function:

Catalyzes the cross-linking of proteins, such as WDR54, and the conjugation of polyamines to proteins.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the transglutaminase superfamily. Transglutaminase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.